EFFECT OF AUXIN AND CYTOKININ FOR INVITRO REGENERATION OF PRUNUS DOMESTICA L. VAR. PIONEER EXPLANTS: AN ECONOMICALLY IMPORTANT FRUIT TREE
Journal Title: International Journal of Bio-Technology and Research (IJBTR) - Year 2017, Vol 7, Issue 2
Abstract
This study was conducted to know the effect of Auxin and Cytokinin type and its concentration for invitro propagation of Prunus domestica L.var. Pioneer explants. Pioneer explants were sterilized by using different concentration of sodium hypochlorite and mercuric chloride, and then cultured on Murashige and Skoog media supplemented with different concentration of BAP or KIN alone and in combination with IBA. The shoots were transferred to half strength MS medium supplemented with varying concentrations of IBA or IAA for root growth and development. Explants disinfected with sodium hypochlorite (2%) for 15 minutes and mercuric chloride (0.1 %) for 7 minutes has highest significant survival value. The highest growth and development rate of shoots was obtained on media with BAP and poor multiplication was achieved on media with KIN. From different combinations of KIN or BAP with IBA, the highest shoot induction was observed on MS media supplemented with 0.5 mg/L BAP in combination with 0.1 mg/L IBA with an average number of 3.18 ± 0.58 shoots per explants and 3.36 ± 0.29 cm average shoot length. The highest rooting was observed on 1.0 mg/L IBA with an average number of 4.05 ± 1.2 roots per shoot and 3.66 ± 0.1 cm average root length.
Authors and Affiliations
EYOB KASSAYE WOLELLA, BERHANU DAGNAW
Keywords
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