Effect of caloric restriction on liver function in young and old ApoE/LDLr-/- mice
Journal Title: Roczniki Państwowego Zakładu Higieny - Year 2018, Vol 69, Issue 1
Abstract
Background. Caloric restriction (CR) leads to decrease metabolic intensity, which results in a reduction of oxygen consumption and the amount of free radicals. This can affect the function of the liver. Studies show that caloric restriction does not alter or significantly increase the enzyme activity associated with gluconeogenesis, but the effect was different according to the age of the model animals. Objective. The aim of the study was to determine the effect of caloric restriction on liver function in young and old ApoE/ LDLr-/- mice. Material and methods. Dietary experiments were performed on 2 and 5 month old male ApoE/LDLr-/- mice. Animals were divided into 3 experimental groups (n=6) and fed AIN’93G diet for 8 and 5 weeks, respectively. Control animals were fed ad libitum (AL) and housed in a colony cages. These animals were checked for dietary intake. The second group were also fed ad libitum but the animals were kept individually in cages (stress AL- sAL). Similarly to sAL group, the animals from the CR group were kept individually but received a 30% less diet compared to AL group. At the end of the experiment animals were euthanized and the blood, liver and adipose tissue have been collected. Alanine aminotransferase (ALT) as well as aspartate aminotransferase (AST) were measured in plasma. Fatty acid profile was evaluated (relative %) in adipose tissue (GC-MS). Liver’s stetosis was assessed. Results were analyzed statistically (ANOVA, STATISTICA v.10.0). Results. CR ApoE/LDLr-/- mice showed significantly lower body weight compared to animals, both AL and sAL. There were no significant differences between ALT and AST in both younger and older animals. However, negative tendencies were more pronounced in younger animals. In young animals CR significantly increased liver weight compared to AL (4.14 vs 3.73g/100g). In adipose tissue fatty acid profile differed in CR mice compared to control in young animals. Conclusions. Caloric restriction did not affect liver enzymes in mice. Caloric restriction showed similar but not identical metabolic activity in young and old mice.
Authors and Affiliations
Renata B. Kostogrys, Magdalena Franczyk-Żarów, Angelika Manterys, Iwona Wybrańska
Keywords
Related Articles
- EP ID EP497003
- DOI -
- Views 92
- Downloads 0
Assessment of physical activity, energy expenditure and energy intakes of young men practicing aerobic sports
Background. Adequate nutrition and energy intake play key rule during the training period and recovery time. The assessment of athlete’s energetic needs should be calculated individually, based on personal energy expendi...
Estimation of dietary supplements intake in a selected group of women over 50 and the risk assessment of interactions between the ingredients of dietary supplements and drugs
Background. Concurrent use of dietary supplements and drugs may result in omplications of pharmacotherapy due to possible interactions between their ngredients. Objectives. The aim of the survey was to estimate the in...
Awareness of factors affecting osteoporosis obtained from a survey on retired Polish subjects
Background. Osteoporosis is a growing problem facing modern society and currently poses one of the most serious health challenges. It is a progressive skeletal disease characterised by low bone mineral density whose deve...
Studies on the quality of nutrition in women with polycystic ovary syndrome (PCOS)
Background. Polycystic ovary syndrome (PCOS) manifests itself with various symptoms, therefore it interests representatives of many medical specializations: general practitioners, gynecologists, endocrinologists, dermato...
Coffee consumption vs. cancer risk - a review of scientific data
Coffee and its impact on health continue to be the topic of much heated debate. Until recently, coffee consumption has been believed to be associated with adverse effects, mainly cardiovascular problems. However, the vas...