Effect of Cleistanthin A on Voltage Gated Proton Channels of Human Neutrophils
Journal Title: Journal of Clinical and Diagnostic Research - Year 2018, Vol 12, Issue 1
Abstract
ABSTRACT Introduction: Cleistanthus collinus (C. collinus), a well known plant toxin, contains active principles like Cleistanthin A, Cleistanthin B, Cleistanthin C and Diphyllin. Previous human case reports and animal studies have revealed that C. collinus poisoning leads to type I Distal renal tubular acidosis and type II respiratory failure. However, the mechanism of toxicity of this plant is still uncertain. Based on the hypothesis that blockade of proton channels could result in type II respiratory failure, patch clamp experiments were done to see if Cleistanthin A blocked the proton channels. Aim: To record and compare the changes in the magnitude of voltage-gated proton currents in human neutrophils, before and after addition of Cleistanthin A (test) and control solution. Materials and Methods: The test compound Cleistanthin A was isolated by partition chromatography and characterised using thin layer chromatography. Neutrophils were isolated by density gradient centrifugation method. Using voltage clamp protocol, proton currents were recorded before (pre-intervention currents) and after (post-intervention currents) the addition of Cleistanthin A or control solution. The pre and post-intervention current densities for different voltages were compared within the groups (control and test) by Wilcoxon signed-rank test and the percentage current remaining in both the groups were compared using Mann-Whitney U test, p<0.05 was considered significant. Results: Normal proton currents were recorded in human neutrophils. Comparison of the pre and post-intervention current densities within the control and test group revealed a significant depletion effect in the control group but not in the test group. However, comparison of the percentage current remaining after intervention across the groups did not show any significant difference between the control and test groups. Conclusion: Cleistanthin A does not seem to have any significant effect on the voltage-gated proton channels of human neutrophils.
Authors and Affiliations
Rajasegaran Rajalakshmi, Soosai Manickam Amirtham, V Abirami, Sathya Subramani, Praghalathan Kanthakumar
Keywords
Related Articles
- EP ID EP502639
- DOI 10.7860/JCDR/2018/31729.11124
- Views 77
- Downloads 0
Effect of Cycling on Glycaemia, Blood Pressure, and Weight in Young Individuals with Type 2 Diabete
Introduction: Aerobic exercise is associated with significant improvement in glycaemia and weight loss in Type 2 diabetes (T2D). Cycling, a form of aerobic exercise can benefit young (18 to <40 years) individuals with T2...
Environmental and Biological Risk Factors Associated with the Prevalence of Language Delay in Children Upto 6 Years of Age from Rural South India
Introduction: There is large amount of data on the prevalence and risk factors of speech and language delay in children up to three years of age, but the data from three to six years of age group is very limited. Aim: To...
Quality of Working Life among Pharmacists in Vietnam: A Preliminary Study using an Internet-Based Survey
ABSTRACT Introduction: The Quality of Working Life (QWL) is an important factor for achieving high-performance effectiveness from workers. Despite the importance of studying QWL to enhance worker satisfaction and perform...
Agreement of Axial Length Measurements by Three Different Biometric Devices
ABSTRACT Introduction: Accurate Intraocular Lens (IOL) power calculation is crucial for spectacle independence and patient satisfaction post cataract surgery. Of the various parameters measured to calculate IOL power, Ax...
Non-Infiltrating Angiolipoma of Floor of Mouth-A Rare Case Report and Literature Review
Angiolipoma, a subtype of lipoma is a benign tumour in which adipose tissue and vascular component are intermingled. The tumour occurs commonly in the trunk and extremities and very rarely seen in maxillofacial region. H...