Effect of Comparison between Qingchangligan Granules and Qingchangligan Decoction on D-GalN/LPS-Induced Liver Injury in Mice
Journal Title: Diseases & Research - Year 2022, Vol 2, Issue 2
Abstract
Ethnopharmacological relevance The Qingchangligan formula has two dosage forms, granules and decoction. Both of them have the same prescription; however, whether their protective effects are different remain elusive. Aim of the study To compare and evaluate the protective effect of the granules and decoction of Qingchangligan formula on acute liver failure (ALF) mice induced by D-galactosamine (D-GalN) and lipopolysaccharide (LPS). Materials and Methods ALF was induced by intraperitoneal injection of D-GalN (700 mg/kg) plus LPS (10 μg/kg). The granules and decoction of Qingchangligan formula were administered to mice in the same dose and at the same time point, respectively, with three doses of 50 mg/kg (on day 1, day 2, and day 3) prior to D-GalN/LPS injection by intragastric administration. The mice in different groups were sacrificed at 6 h after D-GalN/LPS injection, and liver samples and blood were collected for analysis. Results The different processed methods for the Qingchangligan resulted in a little component difference between the granules and decoction: the content of some ingredients, including Catalpol, Caffeic acid, Naringin, Emodin and Chrysophanol in granules, are higher than in decoction. Both of the two Qingchangligan formulas administration ameliorated liver injury, as evidenced by the decreased the lethality in ALF mice, the reduced transaminase levels and well-preserved liver architecture. However, the granules seemed to be more effective than the decoction in the ALF model. Mice pretreatment with the Qingchangligan formula reduced the expression of inflammatory cytokines and promoted autophagy in ALF model. Moreover, the effect on inflammation and autophagy of granules is more obvious than the decoction. Conclusions Our findings demonstrated that the granules and decoction of Qingchangligan formula both exert a protective effect against the pathophysiology of ALF, especially the granules is more efficacious, which provide a rationale for using of the different formulations of Qingchangligan formula.
Authors and Affiliations
Xiangying Zhang, Tingting Ma, Feng Ren, Xiuhui Li
Keywords
Related Articles
- EP ID EP740149
- DOI 10.54457/DR.202202003
- Views 31
- Downloads 0
An Analysis of Gut Bacterial and Fungal Community Interactions in Saxifraga stolonifera Curt.-Treated Mice
Backgrounds Saxifraga stoloniferaCurt. is valued for its great medicinal potentials, especially for anti-inflammatory activity. The gut microbiome is gradually regarded as a pivotal part that contributes to the therapeut...
A Commentary on the Natural Disease Progression Sequence of Chronic Hepatitis B
Hepatitis B virus (HBV), an ancient family of hepatotropic DNA viruses with origins dating back millions of years, remains a major burden to human health today, although hepatitis B vaccines have dramatically reduced the...
Anti-Cancer Agents Work in Antagonism with Inhibitors of HSP 72
Purpose The heat shock genes are evidenced to be active in the migration and are major promoters of several human malignancies. The inclined expression of HSP 72 proteins play an inevitable role in protecting cancer cell...
Roles of Toll-like Receptor 3 in Intrauterine Transmission of Hepatitis B Virus in Mothers with High Viral Load
Backgrounds Despite passive and active immunization, perinatal mother-to-infant transmission (MTIT) of hepatitis B virus (HBV) still occurs in women with high levels of viremia. Thus, understanding the mechanisms of MTIT...
Effect of Comparison between Qingchangligan Granules and Qingchangligan Decoction on D-GalN/LPS-Induced Liver Injury in Mice
Ethnopharmacological relevance The Qingchangligan formula has two dosage forms, granules and decoction. Both of them have the same prescription; however, whether their protective effects are different remain elusive. Ai...