Effect of curcumin on rat sperm morphology after the freeze-thawing process
Journal Title: Veterinary Research Forum - Year 2013, Vol 4, Issue 3
Abstract
Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-thawing process. Sperm collected from 10 adult rats were divided into two groups (n=10 for each group): control and a test group supplemented with 2.5 mM curcumin. After freezing-thawing, the number of spermatozoa, motility, viability, total antioxidant capacity (TAC), and DNA integrity of the sperm were analyzed. Motility, viability and DNA integrity of sperm were significantly preserved in treatment groups compared to the control (p < 0.05) after freezing-thawing. Following cryopreservation, TAC was significantly preserved in thawing semen supplemented with curcumin compared to the control group (p<0.05). Based on our results, it is concluded that curcumin addition during freezing resulted in positive effects on sperm parameters after thawing in adult rats.
Authors and Affiliations
Ali Soleimanzadeh, Adel Saberivand
Keywords
Related Articles
- EP ID EP120488
- DOI -
- Views 113
- Downloads 0
Effects of BIO on proliferation and chondrogenic differentiation of mouse marrow-derived mesenchymal stem cells
In vitro expansion of mesenchymal stem cell (MSCs) into large number is necessary for their application in cell-based treatment of articular cartilage defects. On the other hand, some studies have indicated that BIO (6-...
Clinical, laboratory and pathological findings in sub-acute monensin intoxication in goats
Toxic effects of monensin, a polyether antibiotic mainly used as a coccidiostat, have been described in a wide range of animals. The present study was performed to investigate the toxic effects of monensin in goats. Seve...
Study on effect of salinity level of water on electrocardiogram and some of blood serum minerals in grass carp, Ctenopharyngodon idella
In this study the effects of salinity on the electrocardiogram and some of blood serum minerals in grass carp were investigated. For this purpose, grass carp were exposed to concentrations of 4, 8 and 12 g L-1 salinities...
Effect of vitamins C or E supplementation to Tris diluent on the semen quality of Awassi rams preserved at 5 ˚C
The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructose-egg yolk diluent to increase Awassi ram sperm storage period at 5 ˚C. Semen samples from six mature Awassi rams were used in thi...
Unilateral orchidectomy in donkey (Equus asinus): Evaluation of different surgical techniques, histological and morphological changes on remaining testis
Unilateral orchidectomy (UO) is required when further breeding potential is important. It is sometimes necessary to remove a single testis in a matured stallion for therapeutic reasons. In this study, twelve donkeys were...