Effect of Different Doses of Pregabalin on Erythrocyte Deformability in Rats with Lower Limb Ischemia Reperfusion Injury

Journal Title: International Journal of Anesthesiology & Research (IJAR) - Year 2017, Vol 5, Issue 3

Abstract

Aim/Introduction: Acute ischemia reperfusion (IR) injury observed in the lower extremities occurs especially when a temporary cross-clamp is applied to the abdominal aorta during aortic surgery. Preoperative pregabalin has been used as a part of multimodal analgesia in postoperative pain treatment in recent years. Pregabalin has become one of the increasingly common agents in postoperative analgesia. In this study, we aimed to investigate the effect of pregabalin on erythrocyte deformability in rats undergoing IR. Materials and Methods: 24 male Wistar albino rats weighing between 200-250 g were used in the study. Rats were randomly divided into 4 groups of 6 rats each (Control, Ischemia-Reperfusion (IR), IR-Pregabalin 50 mg (50 mg.kg-1), IRPregabalin 200 mg (200 mg.kg-1). Pregabalin was administered intraperitoneally 30 min before the procedure. An atraumatic microvascular clamp was placed across the infrarenal abdominal aorta in the IR groups. Following 120 min of ischemia, the clamp was removed and reperfusion was continued for 120 min. All rats were euthanized by intraperitoneal administration of ketamine (100 mg•kg−1) and taking blood from the abdominal aorta. Erythrocytes were seperated from heparinized whole blood samples. Deformability measurements were made in erythrocyte suspensions in phosphate buffered saline. A constant flow filtrometer system was used to measure erythrocyte deformability and relative resistance was calculated. Results: It was found that the formation of ischemia reperfusion increases the relative resistance according to the control group (p < 0.0001). It was determined that application of pregabalin 50 or 200 mg did not change erythrocyte deformability in ischemia reperfusion-induced rats (p = 0.632, p = 0.811). Conclusion: The administration of 50 or 200 mg of pregabalin has no negative effect on the erythrocyte deformability in ischemia reperfusion-induced rats. We think that pregabalin can be safely used for analgesia in the cases of IR. However, these findings should be supported by clinical and experimental studies carried out in more detailed and broader series.

Authors and Affiliations

Mustafa ARSLAN

Keywords

Related Articles

Asystole During Airway Manipulation In Neurosurgical Patient: Causes And Management

Hemodynamic disturbances in neurosurgical patients may be attributed by various neurogenic causes mainly raised intracranial pressure (ICP); however, cardiovascular perturbations during airway management in such patients...

Dexmedetomidine-based Total Endovenous Anesthesia in Radical Surgery for Breast Cancer. An observational case series study involving 10 patients

Introduction: Ambulatory treatment of breast cancer is limited by vomiting and post-operative pain, and general anesthesia is implicated in these effects. Dexmedetomidine supplies analgesia and sedation without respirato...

The Effect of Intravenous Fentanyl and Bupivacaine Infiltration on the Hemodynamic Respons During Mayfield Skull Clamps Fixation in Neurosurgery

Background: The aim of our study was to compare the effects of three techniques for preventing the hypertensive response to the insertion of Mayfield skull pins (MSP): group F (fentanyl), group B (bupivacaine) and FB (co...

To Compare the Efficacy of Phenylephrine and Mephentermine in Maintaining Arterial Pressure During Spinal Anaesthesia in Infra Umbilical Surgeries

Introduction: It is important to find an effective method to prevent and treat hypotension during subarachnoid block. Hence we compare the bolus doses of Phenylephrine and Mephentermine to treat hypotension post subarach...

Does the Administration of Preoperative Midazolam Assist in Maintaining Blood Glucose Norms in the Non - Diabetic Patient during the Perioperative Period?

Introduction: Surgery induces stress response, which can cause elevated blood glucose level. In addition to tissue injury and inflammation, anxiety can contribute to the stress response. We measured the effect of preoper...

Download PDF file
  • EP ID EP279599
  • DOI 10.19070/2332-2780-1700085
  • Views 114
  • Downloads 0

How To Cite

Mustafa ARSLAN (2017). Effect of Different Doses of Pregabalin on Erythrocyte Deformability in Rats with Lower Limb Ischemia Reperfusion Injury. International Journal of Anesthesiology & Research (IJAR), 5(3), 414-416. https://europub.co.uk/articles/-A-279599