Effect of Different Doses of Pregabalin on Erythrocyte Deformability in Rats with Lower Limb Ischemia Reperfusion Injury
Journal Title: International Journal of Anesthesiology & Research (IJAR) - Year 2017, Vol 5, Issue 3
Abstract
Aim/Introduction: Acute ischemia reperfusion (IR) injury observed in the lower extremities occurs especially when a temporary cross-clamp is applied to the abdominal aorta during aortic surgery. Preoperative pregabalin has been used as a part of multimodal analgesia in postoperative pain treatment in recent years. Pregabalin has become one of the increasingly common agents in postoperative analgesia. In this study, we aimed to investigate the effect of pregabalin on erythrocyte deformability in rats undergoing IR. Materials and Methods: 24 male Wistar albino rats weighing between 200-250 g were used in the study. Rats were randomly divided into 4 groups of 6 rats each (Control, Ischemia-Reperfusion (IR), IR-Pregabalin 50 mg (50 mg.kg-1), IRPregabalin 200 mg (200 mg.kg-1). Pregabalin was administered intraperitoneally 30 min before the procedure. An atraumatic microvascular clamp was placed across the infrarenal abdominal aorta in the IR groups. Following 120 min of ischemia, the clamp was removed and reperfusion was continued for 120 min. All rats were euthanized by intraperitoneal administration of ketamine (100 mg•kg−1) and taking blood from the abdominal aorta. Erythrocytes were seperated from heparinized whole blood samples. Deformability measurements were made in erythrocyte suspensions in phosphate buffered saline. A constant flow filtrometer system was used to measure erythrocyte deformability and relative resistance was calculated. Results: It was found that the formation of ischemia reperfusion increases the relative resistance according to the control group (p < 0.0001). It was determined that application of pregabalin 50 or 200 mg did not change erythrocyte deformability in ischemia reperfusion-induced rats (p = 0.632, p = 0.811). Conclusion: The administration of 50 or 200 mg of pregabalin has no negative effect on the erythrocyte deformability in ischemia reperfusion-induced rats. We think that pregabalin can be safely used for analgesia in the cases of IR. However, these findings should be supported by clinical and experimental studies carried out in more detailed and broader series.
Authors and Affiliations
Mustafa ARSLAN
Keywords
Related Articles
- EP ID EP279599
- DOI 10.19070/2332-2780-1700085
- Views 99
- Downloads 0
Acromegaly, Endocrine Dysfunction and Polyostotic Fibrous Dysplasia Associated With Mccune-Albright Syndrome: Anesthetic Considerations
McCune-Albright Syndrome is a rare disorder affecting the skin, bones and endocrine tissues. It is characterized by the presence of café au lait spots, polyostotic fibrous dysplasia, and autonomous endocrine hyperfunctio...
Our Experience of using Caudal Anesthesia in "Small" Surgical Interventions in Newborns
Aim of the Study: To study the effectiveness of anesthesia with caudal administration of ropivacaine in combination with intravenous administration of propofol in new borns with "small" surgeries. Material and Methods...
Percutaneous Dilatational Tracheostomy: ULTRAperc® Single Stage Dilator Technique Versus Ciaglia Blue Dolphin® Balloon Dilatation Technique for Intubated Unweanable ICU Patients
Background: Percutaneous tracheostomy is currently one of the most common procedures performed in ICU. It has demonestrated provide numerous advantages over the classical surgical tracheostomy. The aim of this study is t...
Does the Administration of Preoperative Midazolam Assist in Maintaining Blood Glucose Norms in the Non - Diabetic Patient during the Perioperative Period?
Introduction: Surgery induces stress response, which can cause elevated blood glucose level. In addition to tissue injury and inflammation, anxiety can contribute to the stress response. We measured the effect of preoper...
Pediatric Renal Transplantation in Children with Weight 20kg or Less: A Single-Center Experience
Background: Renal transplantation (RT) is the treatment of choice for children with Chronic Kidney Disease (CKD). This technique benefits survival and quality of life. Long-term outcomes in pediatric transplantation have...