Effect of different drying conditions for the viability of Candida albicans present on carriers
Journal Title: Roczniki Państwowego Zakładu Higieny - Year 2014, Vol 65, Issue 1
Abstract
Background. Problems in substantial under recovery of Pseudomonas aeruginosa and Candida albicans from carriers have been demonstrated for laboratories performing phase 2, step 2 efficacy tests on disinfectants relative to levels required by the EN 13697 standard. It was thus necessary to determine recoveries of these microorganisms following procedural losses incurred during drying and to optimise drying conditions such that recoveries then complied with the standard. Objectives. The aim of the study was to establish optimal drying conditions for the recovery of Candida albicans ATCC 10231 from carriers. Material and Methods. The evaluation was performed according to the EN 13697:2001 standard procedure. A test suspension of Candida albicans and interfering substance were inoculated onto the surface of carriers (2 cm diameter stainless steel discs) and then dried under different conditions consisting of: a 37°C incubation with and without an incubator fan as well as at 23°C (room temperature) in a laminar air flow cabinet. Carriers were dried until the surfaces appeared visibly dry and the number of surviving organisms then recovered from the surface were quantified. The following were calculated for colony forming units (cfu); N (log10 cfu in a 0.05 ml test suspension), NC (the control log10 cfu in neutralizing medium), Nts (cfu numbers remaining on the surface) and the N-NC difference which should not exceed 2 log10 when microorganism recoveries are adequate and without any toxicity effects of the neutralising medium. Experiments was conducted using validating procedure (NC) which is performed with distilled water. Results. Drying at 37°C adversely affected the survival of Candida albicans and prevented the levels of microbial recovery from carriers to reach those specified by the EN 13697 standard. However, drying at around room temperatures of 23°C reduced Candida albicans mortality and increased recoveries from the carrier to levels compliant with the standard, where the N-NC differences were not greater than 2 log10. Conclusions. The viability of Candida albicans ATCC 10231 is sufficiently improved when carriers are dried at 23°C, even if the drying time exceeds 60 minutes. The density of the initial test suspension (N) should also be increased.
Authors and Affiliations
E. Röhm-Rodowald, A. Chojecka, B. Jakimiak, O. Wiercińska, K. Kanclerski
Keywords
Related Articles
- EP ID EP246773
- DOI -
- Views 116
- Downloads 0
Health risk of exposure to bisphenol A (BPA)
Bisphenol A (BPA) belongs to chemicals that are produced in large quantities worldwide. It is commonly used as monomer in polycarbonate synthesis, plasticizer in the production of epoxy resins, as well as an additive for...
Effect of conjugated linoleic acid and different type of dietary fat on serum lipid profile, liver enzymes activity and oxidative stress markers in Wistar rats
Background. Nutritional recommendations emphasize the need to limit consumption of saturated fatty acids and to increase the intake of polyunsaturated fatty acids in the prevention of non-communicable chronic diseases, p...
Evaluation of communication and acceptance of the patients by medical personnel
Background. The low level of patient satisfaction recorded in many studies and, at the same time, the level of frustration and burnout, disclosed by medics in the perception of the patient as a ‘problem’, incline to look...
Determination of chloramphenicol in milk powder using liquid-liquid cartridge extraction (Chem Elut) and liquid chromatography-tandem mass spectrometry
Background. The European Union prohibits the use of chloramphenicol (CAP) as a veterinary drug in food-producing animals. Nevertheless, CAP have been detected in milk products (liquid milk and milk powder). Therefore, it...
Selected indices of health status in preschool children from Pila and their families as a risk factor of diet-related diseases
Background. Diet-related diseases have their origin as early as in childhood; therefore, preventing them should start from the first years of life. Each assessment of dietary intake should be preceded by assessment of he...