Effect of different extenders on recovery and storage of epididymal ram spermatozoa
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2016, Vol 8, Issue 2
Abstract
This experiment was conducted to determine effect of different extender on recovery and storage of epididymal ram spermatozoa. Testes were obtained from eight mature rams which were slaughtered at four different occasions, a pair in each session. Caudal epididymis was isolated and divided to four equal parts. Spermatozoa recovery from each part of the caudal epididymis was performed by milk (M), Tris (T), Tris plus caproic acid (T-C) and sodium citrate (S) extenders. Spermatozoa in each extender were divided into four parts and then the amount of 0 (E0), 5 (E5), 10 (E10) and 15% (E15) egg yolk were added. Samples were chilled to 4 °C and stored for 7 days. The evaluation of sperm motility, viability, membrane integrity and acrosome integrity were performed daily. The results showed that there was interaction between egg yolk and storage time and also egg yolk and extender on sperm motility (p < 0.05). Sperm motility was highest at 15 % egg yolk (25.6 %) on 7 d (p < 0.05). Sperm motility was higher in ME15 (57.8 %) and T-CE15 (62.3 %) than TE15 (53.4 %) and SE15 (41.9 %; p < 0.05). There was interaction among egg yolk, extender and storage time on acrosome integrity, membrane integrity and sperm viability (p < 0.05). On 7 d, there were no differences among ME15, T-CE15 and TE15 on sperm viability and membrane integrity. Acrosome integrity was higher in T-CE15 (75.25 %) than ME15 (62.5 %) on 7 d (p < 0.05). Therefore, Tris caproic extender was superior to other extender for recovery and storage of ram epididymal spermatozoa.
Authors and Affiliations
Mohammad Roostaei-Ali Mehr,Mohammad Atayi,
Keywords
Related Articles
- EP ID EP722433
- DOI -
- Views 37
- Downloads 1
Histopathologic report of infestation by Centrocestus formosanus in Iranian grass carp and common carp
Flukes cause severe and lethal diseases in various animals comprising fish. Both adult and larval stages are found in fish. Centrocestiasis is an infection of the fish gills by heterophyid trematode Centrocestus formosan...
Structure of pancreas in Palam Dove (Streptoplia selegalensis)
The purpose of this study was to have a general view of pancreas in one species of pigeon.Some disorders affect avian pancreas.Pancreatitis and Pancreatic degeneration have been described in many infections and deficien...
Isolation, Antimicrobial Resistance, and Virulence Genes of Thermophilic Campylobacter Species from Backyard Ducks in Amol, Northern Iran
Domestic poultry are considered natural reservoirs for the transmission of Campylobacter spp., mainly C. jejuni and C. coli, to other birds and humans. This study aimed to determine the Campylobacter infection status in...
Milk MycofloraSurvey of Dairy Cows with or without Mastitis
Mastitis in cattle is a serious problem which causes considerable economic losses in the dairy cattle herds. The aim of this survey was to identify mycoflorain milk of healthy, clinical and subclinical mastitis of lactat...
Necrotic ulcerative dermatitis due to simultaneous infections of Malassezia and Microsporum gallinae in a pigeon (Columba livia domestica)
A homing pigeon (Columba livia domestica) was referred with the presence of ulceration, bleeding, loss of feathers and scabs and crust in the head. Mycological evaluation was shown simultaneous infections by Malassezia a...