Effect of different factors on in vitro growth and shoot proliferation of guava (Psidium guajava L.) cv. Allahabad Safeda
Journal Title: Journal of Applied and Natural Science - Year 2016, Vol 8, Issue 4
Abstract
In the present experiment an attempt has been made to optimize the effect of different medium, levels of sucrose, pH, adenine sulphate and light intensity for culture establishment and shoot proliferation in guava using nodal segment explants. Culture establishment was greatly influenced by media types. Maximum establishment of explants (74.57%) was recorded on MS medium supplemented with 2.0 mg/l BAP + 0.2 mg/l IBA. In proliferation study, the maximum shoot proliferation was observed in MS + 1 mg/l BAP + 0.25 mg/l GA3. Sucrose 3% was found to be more favorable for maximum proliferation and growth of shoots moreover, it was reduced gradually as increased or decreased levels of sucrose from 30 g/l. among the various pH levels tested, pH 5.5 recorded maximum number of shoots (8.08) and maximum length of shoots (3.75 cm).In proliferation medium the length of shoot, numbers of shoots and growth rate were increased as increased the adenine sulphate level in the medium. Maximum proliferation was observed on 160 mg/l adenine sulphate in the medium. High light intensity 3000 lux was found to be most suitable for proper growth of regenerated shoots. Low light intensity (1000 lux) resulted in stunted growth. All the above factors significantly influenced shoot multiplication and growth. Thus, optimization of these factors showed significantly increased number of shoots and rapid multiplication. This could be useful for the in vitro production of cost effective healthy planting material of guava
Authors and Affiliations
Smita Kadam, R. M. Patel, Pushpraj Singh
Keywords
Related Articles
- EP ID EP285630
- DOI -
- Views 73
- Downloads 0
Germination response of Treculia africana (Dacne) seeds in relation to moisture content, storage method and its duration
Edible seeds of forest tree crop may be unavailable in sufficient quantity for tree planting projects and so there is need for storage. The germination response of Treculia africana seeds to storage method, duration and...
Ion chromatography determination of anionic change in surface and ground water due to industrial effluents in Jammu (J&K), India
In present investigation of anionic (fluoride, chloride, bromide, phosphate and sulphate) change in surface and ground water due to industrial effluents by ion chromatography technique has been determined in area of Jamm...
Utilization of extracted substance from Indian almond leaves, Terminalia catappa L. for preparation of folk medicinal cream for the use of skin protection
The aim of this work was to study the method to prepare the folk medicinal cream for the use of skin protection from the extracted substance of Indian almond leaves, Terminalia catappa L. in 4 periods as young green leaf...
Ability of arbuscular mycorrhiza to promote growth of maize plant and enzymatic activity of an alluv
A pot experiment was conducted to evaluate the response of selected species of mycorrhizae for root colonization and phosphorus uptake by maize in an alluvial soil. Of all the species of mycorrhizae taken under considera...
Performance of some apricot (Prunus armeniaca L.) germplasm accessions for fruit quality traits in Himachal Pradesh
The present investigation was carried out in field gene bank of NBPGR Regional Station Phagli, Shimla during the year 2014-15. Twenty accessions of Prunus armeniaca L. namely St. Ambroise, Wenatchee, Nugget, Stirling, Na...