Effect of Enrichment of In Vitro Fertilization Medium with Cysteine on Fertilization and Embryo Development in Buffaloes
Journal Title: International Journal of Veterinary Health Science & Research (IJVHSR) - Year 2017, Vol 5, Issue 6
Abstract
The current study aimed to elucidate the effect of adding various concentrations of cysteine to the in vitro fertilization culture medium on the fertilization rate and following embryonic development of buffalo oocytes. In vitro matured oocytes were fertilized using different concentrations of cysteine (0.5, 1.3 mM) in the presence of 5.0 mM caffeine. After in vitro fertilization, fertilization rate, cleavage rate and developmental rates till the blastocyst stage have been evaluated. The present results revealed that supplementation of the fertilization media with 0.3 Mm cysteine enhanced significantly (P<0.05) the fertilization rate and the development rates till the morula and blastocyst stages (53.45% ± 6.61%, 19.18% ± 4.64% and 13.70% ± 4.05%, respectively) compared to those of the control groups (30.65% ± 5.90%, 5.56% ± 3.15% and 1.85% ± 1.85 %, respectively). The current results demonstrated that addition of 0.3 mM cysteine to a fertilization medium had positively improved in vitro fertilization rates and supported embryonic progress till the blastocyst stage of buffalo oocytes.
Authors and Affiliations
Samy Moawad Zaabel
Hygienic Handling and Processing of Raw Beef Meat at Slaughter Houses and Meat Stalls in Gojjam Area, Ethiopia
A cross sectional study was conducted to assesses human resource capabilities, sanitation of slaughter houses and processing materials; and hygienic indicator microorganisms and specific foodborne pathogens. Observation...
An Abattoir Based Study on Brucellosis, Bovine Tuberculosis and Paratuberculosis in Buffaloes and Cattle at Faisalabad, Pakistan
A study was carried out to investigate the prevalence of these diseases at Faisalabad abattoirs in buffaloes and cattle. For this purpose, 400 consecutive animals, including 200 buffaloes and 200 cattle were included in...
Use of a Topical Spray Made of Essential Oils and Essential Fatty Acids from Plant Extracts for the Treatment of Microbial Infections in Dogs: An Opened Multicentric Study of 47 Cases
Bacterial pyoderma and Malassezia dermatitis are frequent dermatosis in dogs, for which the use of systemic courses of respectively antibiotics and/or anti fungals is needed. However, as the risk of emergence of antimicr...
Metastatic Adenocarcinoma of Unknown Primary Origin Presenting with Severe Lumbar Neuropathic Pain in a Dog
An eleven year-old, male, beagle was presented with a two-month history of progressive stiff gait and spinal hyperalgesia. On the basis of clinical and magnetic resonance images features, an extensive bilateral paraspina...
A Study of the Effect of Vitamin C and Ocimum Sanctum Supplementation on Antioxidant Enzyme Levels in Broilers Under Heat-Stress
This experiment was conducted in heat-stressed broiler chicken to evaluate the effect of dietary supplementation of Vitamin C and Sanctimonious on antioxidative enzyme levels. A total of forty broiler chickens of day old...