Effect of follicle stimulating hormone and testosterone on viability rate of cryopreserved spermatogonial stem cell after thawing
Journal Title: Iranian Journal of Veterinary Science and Technology (IJVST) - Year 2013, Vol 5, Issue 1
Abstract
Stem cells are generally defined as clonogenic cells capable of both self-renewal and differentiation. Probably the best method for long-term preservation of spermatogonial stem cells is cryopreservation. In this study, effects of Follicle Stimulating Hormone and Testosterone on viability rate of cryopreserved spermatogonial stem cell after Thawing were investigated. Sertoli and spermatogonial cells were isolated from 3-5 months old calves. Cocultured sertoli and spermatogonial cells were treated with Follicle Stimulating Hormone and Testosterone in treatment groups before cryopreservation. Results indicated that Follicle Stimulating Hormone increased viability rate of cryopreserved spermatogonial cells in comparison with Testosterone and control group. In conclusion, using Follicle Stimulating Hormone provided proper bovine spermatogonial stem cell culture medium for in vitro culture and cryopreservation of these cells.
Authors and Affiliations
Reza Narenji Sani,Parviz Tajik,Mohammad Hasan yousefi,Mansoureh Movahedin,Shiva Shafiei,
Keywords
Related Articles
- EP ID EP722497
- DOI -
- Views 45
- Downloads 1
Detection of Giardia duodenalis antigen in companion rabbits of Ahvaz district, South-West of Iran
Giardia duodenalis is an important zoonotic protozoon, found in the small intestine of humans and mammals. There is very little information available regarding rabbits infected with Giardia in Iran. The objective of the...
Evaluation of Antibacterial Activity of cLFchimera and its Synergistic Potential with Vancomycin against Methicillin-Resistant Staphylococcus aureus
Frequent and unlimited use of antibiotics caused the development of antibiotic resistance by microorganisms. Therefore, there is an argent need to discover novel antibacterial agents or a combination of agents as a safe...
The epidural analgesic effects of Meloxicam in dogs
Epidural analgesia is an effective and frequent method used to induce analgesia in perineal region during orthopedic or obstetric procedures of pain management. 18 healthy mixed- breed dogs, were randomly divided into...
A survey on the gastrointestinal parasites of exotic companion species in Tehran, Iran
Exotic pet owners, ranging from small mammals to reptiles, comprise a considerable portion of veterinary clients. Parasitic infections are a threat both for the animal health and the health of the owner. This study aimed...
Long-term outcome after surgical treatment of a congenital flexor tendon deformity in a pony
Equine congenital or acquired flexor tendon deformity can occur immediately after birth or at any stage in the first 24 months of life. The long term prognosis after treating a severe flexor tendon deformity in horses ma...