Effect of freezing and thawing rates on sperm motility in Bocachico Prochilodus magdalenae (Pisces, Characiformes)
Journal Title: Revista MVZ Cordoba - Year 2013, Vol 18, Issue 1
Abstract
Objective. To determine the freezing and thawing rates necessary to maintain sperm viability during cryopreservation of Bocachico semen. Materials and methods. Four interactional treatments were implemented between two freezing (rapid and slow) and two thawing (rapid and slow) curves, in a 2x2 factorial as follows: rapid freezing-rapid thawing, rapid freezing-slow thawing, slow freezing-rapid thawing, and slow freezing-slow thawing. After thawing by Sperm Class Analyzer (SCA) curvilinear velocity (VCL) and straight-line (VSL) (μm sec-1) were analyzed; total, rapid, medium, and slow motility, were compared among treatments. Results. The rapid freezing-slow thawing treatment was lethal for all variables of velocity and motility, causing a significant (p<0.01) post-thaw inmotility of 100%. The slow freezing-rapid thawing interaction had a significantly higher effect than the other treatments (p<0.05), particularly on variables such as rapid motility (10.1 ± 1.1%), medium motility (30.16 ± 4.1%), and curvilinear velocity (51.5 ± 4.75 μm sec.-1) also decreased the percentage of sperm with slow motility (41.7 ± 4.45%). Independently of the applied thawing rate, the freezing rate generated the main significant effect on total motility. Conclusions. It is possible to conclude that the interaction effect between freezing and thawing rates is nil (except for slow motility) during cryopreservation process. However, the independent effects of these factors (main effects) on remaining motility variables are positively significant and decisive to the maintenance of these features, especially the freeze factor (when it is slow). This becomes the first successful report of sperm cryopreservation from Bocachico Prochilodus magdalenae in the world and may be used in conservation programs for this endangered species.
Authors and Affiliations
José G. Martínez, M. Sc| Universidade Federal do Amazonas, Instituto de Ciências Biológicas, Laboratório de Evolução e Genética Animal –LEGAL, Manaus, Brasil., Sandra Pardo C, Ph. D *| Universidad Nacional de Colombia sede Medellín, Facultad de Ciencias Agrarias, Departamento de Producción Animal, BIOGEM, Medellín, Colombia.Corresponding: scpardoc@unal.edu.co
Keywords
Related Articles
- EP ID EP3288
- DOI -
- Views 390
- Downloads 13
Aglepristone efficiency with and without the canine pyometra cloprostenol
Objective. Evaluate the effectiveness of aglepristone with and without cloprostenol for treating canine pyometra. Materials and methods. Ten female French Bulldogs between 3-8 years of age diagnosed with pyometra were di...
Associations between copper concentrations in liver and serum and ceruloplasmin in serum from horses
Objective. The aim of this study is to determine the reference intervals and the associations between the concentrations of liver and blood serum copper (Cu) and serum ceruloplasmin (Cp) in horses. Materials and Methods....
TESTICULAR NEOPLASMS IN DOGS: A CASE OF SERTOLI CELL TUMOR
Testicular neoplasms are not common in domestic animals. Among domestic animals, canines manifest them with more frequency (0.7% to 4.6% of all canine tumors). Some predisposing factors including age and the presence of...
SEASONALITY, CYCLE AND VOLATILITY OF PRICE CATTLE LEVANT MALE IN MONTERIA, COLOMBIA
Objetive. To describe the temporal behavior of prices from livestock young male of first quality in Monteria city, Colombia, marketed by cattle auctions. Materials and methods. Analysis of weekly and monthly prices durin...
Protozoan and metazoan parasites of Nile tilapia Oreochromis niloticus cultured in Brazil
Objective. This study describes the parasitic fauna and relative condition factor (Kn) in Nile tilapia Oreochromis niloticus L. (Cichlidae) from fish farms in the State of Amapá. Material and methods. 123 fish from four...