Effect of Fucoxanthin on PI3K/Akt Signaling Pathway in Human Cervical Cancer HeLa Cells
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2018, Vol 9, Issue 3
Abstract
Objective: To study the effect of Fucoxanthin on PI3K / Akt signaling pathway in human cervical cancer Hela cells. Method: We exposed HeLa cells to different concentrations of Fucoxanthin for 24 hours. The effect of Fucoxanthin on PI3K/Akt signaling pathway related proteins was detected by Western blot. In order to analyze the relationship between nuclear transcription factor activity and Fucoxanthin, the activity of NF-kappa B and AP-1 was determined by luciferase reporter gene assay. Results: Western blot analysis showed that Fucoxanthin inactivated Akt pathway, inhibited Bcl-2 protein level, induced Bax production and caspase-3 cleavage. The Fucoxanthin may inhibit the growth of HeLa cells by inhibiting the PI3K/Akt signaling pathway. Fucoxanthin dose dependently reduced the activation level of NF- kappa B but did not significantly affect AP-1. This indicates that Fucoxanthin inhibits the activation of NF-kappa B in HeLa cells. Conclusion: NF-kappa B may be the mediator of Fucoxanthin-induced apoptosis in HeLa cells. As an ideal target for tumor therapy, NF-kappa B can regulate some genes involved in the development and progression of cervical cancer and inhibit cell apoptosis by activating several anti- apoptosis genes.Epidemiological studies have found that cervical cancer is closely related to human papilloma virus (HPV) infection, probably due to genetic damage of cells and integration of high-risk HPV- DNA [1]. Studies have shown that PI3K/Akt signal is over-activated in various human squamous cell carcinomas associated with HPV infection. This conclusion has been further confirmed in cervical cancer. Abnormal activation of this pathway and HPV-encoded E-series carcinogens have been reported to play a synergistic role in promoting malignant transformation of cervical cells [2]. transfected cervical cancer cells with E5 gene and found that E5 protein could up-regulate the expression of vascular endothelial growth factor by activating PI3K/Akt signal [3]. Transfected cervical cancer HeLa cells with lentiviral vector carrying siRNA of HPV18-E6 gene. It was found that inhibition of E6 protein expression could significantly down-regulate Akt expression at mRNA and protein levels, effectively inhibiting the growth of HeLa cells [4].
Authors and Affiliations
Guo Liu YE, Li Jie JIN, Ling Ling WANG, Dan Li DU
Keywords
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- EP ID EP591782
- DOI 10.26717/BJSTR.2018.09.001819
- Views 146
- Downloads 0
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