Effect of Garcinia Mangostana Linn Fruit Peel Ethanolic Extract on Fibroblast Cell Migration
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2019, Vol 19, Issue 3
Abstract
Wound healing is the survival process against disruptor of living cells or tissues. Many attempts have tried to develop the products for promoting wound healing process. Garcidine®, a hospital formula containing 10% w/v ethanolic extract of Garcinia mangostana Linn fruit peel in propylene glycol base, is the topical anti-septic product for treating open wound. This current study aimed to determine wound healing efficiency of Garcidine® by using fibroblast cell migration model. Cell migration of Garcidine® treated fibroblast cells was compared to that of 10%w/v povidone iodine. The results revealed that Garcidine® was superior to 10% w/v povidone iodine in increasing fibroblast cell migration within 12 hours of treatment. Cytotoxic effect on fibroblast cells was not evidenced in both formulations. Therefore, this finding suggests that Garcinia Mangostana Linn fruit peel ethanolic extract is the promising natural product for treating open wound due to the efficacy in increasing fibroblast cell migration. Wound is the disruption of cellular and anatomical living tissue. It can be produced by chemical, physical and microorganism attacking the tissue and is a major cause of organ abnormal function and disability [1]. Wound healing is a survival process for protecting and regenerating damage tissue. The healing process is triggered since tissue injury; platelets are activated and attracted into the injury area. Platelet aggregation induces the release of clotting factor that results in the deposition of a fibrin clot at the site of injury. Immune cells also interact with fibrin clot and release the cytokines and growth factors such as interleukin-1 beta (IL- 1β), tumor necrosis factor alpha (TNF-α) and transforming growth factor beta (TGF-β). Fibroblasts cells obviously response to TGF-β and then perform collagen deposition for repairing the injury tissue [1,2]. Due to fibroblasts responsibility to collagen production, they are usually studied for estimating wound healing process by using cell migration and proliferation models [3]. Many studies used fibroblast cells for predicting wound healing promotion activity [1,3]. Garcinia mangostana Linn. has many chemical compounds that present various bio activities? There was a study reported on wound healing promotion activity of G. mangostana in Spraque-Dawley rats by inducing skin epithelialization and wound contraction [4]. Moreover, xanthones isolated from G. mangostana presented potent anti-microbial [5,6] and anti-inflammatory activities [7,8]. Both activities play crucial role in wound healing promotion. Garcidine® is a topical anti-septic product containing G. mangostana peel extract in propylene glycol. The formula is formulated according to Thailand National List Essential Medicines recommendation [9]. Current study aimed to investigate wound healing promotion activity of Garcidine® by using fibroblast cell migration model.
Authors and Affiliations
Wudtichai Wisuitiprot, Sutthinee Wisutthathum, Supaporn Pitiporn, Vanuchawan Wisuitiprot, Pakakrong Kwankhao, Neti Waranuch
Keywords
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- EP ID EP621725
- DOI 10.26717/BJSTR.2019.19.003317
- Views 181
- Downloads 0
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