Effect of growth regulators on in vitro regenerative ability of explants of quercus robur l.
Journal Title: Біоресурси і природокористування [Biological Resources and Nature Management] - Year 2017, Vol 9, Issue 3
Abstract
Development of efficient in vitro mass production technology of uninfected planting material of valuable forest-forming woody plants, including English oak (Quercus robur L.), is one of the urgent tasks of today. Domestic and foreign authors note that getting stably growing in vitro culture explants of woody plants of the family Fagaceae Dumort. is quite difficult. The purpose of the research was to determine the effect of growth regulators on in vitro regenerative ability of Q. robur explants. For the studies that were carried in January and February 2016, the embryos with fragments of the endosperm of 120-year-old donor plant were used. The following study methods were exploited: biotechnological (plant tissue culture in vitro, microclonal propagation, callus culture), statistical. It was established that the effective sterilization (above 80 %) of Q. robur embryos was achieved through the use of 0.1 % HgCl2 within 15–16 minutes. The optimal conditions for induction of callus formation in leaf blade tissues of plants Q. robur with a frequency of more than 90 % and active growth were received on MS medium (T. Murashige & F. Skoog) with the addition of 1.0 mg•L-1 BAP (6-Benzyladenine) and 0.5 mg•L-1 NAA (1-Naphthylacetic acid) in an incubator and without light. An active shoots formation in plant explants in vitro was fixed on MS medium modified with 1.0 mg•L-1 2-iP (6-(ɣ,ɣ-Dimethylallylamino) purine) and 20 mg•L-1 of adenine. Further studies are aimed at obtaining stably growing in vitro cultures of Q. robur for mass microclonal propagation
Authors and Affiliations
O. Yu. Chornobrov
Keywords
Related Articles
- EP ID EP257968
- DOI -
- Views 92
- Downloads 0
Modelling of climate change mitigation policies on national scale
The Kyoto Protocol, Paris agreement, concept of sustainable development are designed to solve global environmental problem and to limit the increase in global temperature to no more than 1.5 to 2 degrees Celsius. Integra...
Component approach of landscape aesthetics research
As a result of analytical studies of existing principles and approaches to assessing the aesthetics of landscapes, aspects have been identified regarding which they are systematized: the presence of a person in the envir...
THE RISK OF DOSE LIMITS EXCESS ON THE ECOSYSTEM ELEMENTS CALCULATION USING THE ERICA TOOL
The possibilities of using the ERICA tool software to calculate the dose load on the ecosystem were analyzed. The expediency of using the software to optimize the sampling process in ecosystems with different levels of p...
Monitoring of food, feed, agricultural raw material for genetically modified ingredients in Ukraine
Methodology and test-systems for GM-plant detection by Real-Time PCR were designed. Monitoring of GM-plant distribution in Ukraine was performed. Obtained results showed an urgency of GM-ingredients control in food produ...
Characteristics of new varieties of winter common wheat breeding of the myronivka institute of wheat as the allelic state of the lr34 leaf rust resistance gene
Identification of the allele state Lr34 gene of resistance to the brown rust causative agent by a dominant marker based on polymorphism in 12 exons of caSNP12 in new varieties of soft winter wheat and their investigation...