Effect of hematopoietic stem cell on tumor necrosis factor-毩expression, spiral artery remodeling and placental apoptosis in lead-exposed pregnant mice
Journal Title: Asian Pacific Journal of Reproduction - Year 2017, Vol 6, Issue 4
Abstract
Objective: To evaluate the effectiveness of hematopoietic stem cell (HSC) on TNF-毩 expression, spiral artery remodeling, and placental apoptosis. Methods: The study used pregnant mice divided to five groups of treatment: Group 1, without treatment; Group 2, 1/12 LD50 of lead and Aquadest; Group 3, 1/16 LD50 of lead and Aquadest, Group 4, 1/12 LD50 of lead and 1×107 HSC; and group 5, 1/16 LD50 of lead and 1×107 HSC. Histopathology examination was performed to identify spiral artery remodeling. Immunohistochemistry analysis was conducted to characterize TNF-毩 expression and the apoptosis index. The data from all five groups were compared using the ANOVA test. Results: TNF-毩 expression differed significantly among the groups that were not exposed to lead, exposed to lead and exposed to lead after HSC treatment (P<0.01). There was also a significant difference of spiral artery remodeling between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment (P<0.05). In apoptosis index evaluation there was a significant difference between groups without lead-exposed, lead exposed and lead exposed followed HSC treatment, but not in the groups of 1/16 LD50 of lead followed HSC (P>0.05). Conclusions: In lead-exposed pregnant mice, the administration of hematopoietic stem cell may decrease TNF-毩 expression, spiral artery remodeling, and placental apoptosis index but in the group of 1/16 LD50 of lead and HSC treatment, apoptosis index has no differences with those of control group.
Authors and Affiliations
None Widjiati, Suryo Kuncorojakti, Hendy Hendarto, Viski Fitri Hendrawan, Aulanni’am Aulanni’am
Keywords
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- EP ID EP240843
- DOI 10.12980/apjr.6.20170403
- Views 166
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