Effect of Hormone Priming on Germination Characteristics and Enzyme Activity of Mountain Rye (Secale montanum) Seeds under Drought Stress Conditions
Journal Title: Журнал ÑтреÑÑ-физиологии и биохимии - Year 2013, Vol 9, Issue 3
Abstract
Seed priming techniques have been used to increase germination, improve germination uniformity and seedling establishment under stressed conditions. Seed priming was used in Rye Mountain (Secale montanum) to increase seed germination and tolerance on stress exposure. Rye seeds were treated with various priming agents for different time and temperatures. The effect of priming was assessed on germination characteristics on subsequent exposure to drought (PEG -14 bar) stress for 7 days. Seed priming treatments significantly (p≤ 0.01) affected, Germination percentage (GP), normality seedling percentage (NSP), germination Index (GI), germination uniformity (GU), means time to germination (MTG), coefficient of velocity of germination (CVG), seedling vigour index (SVI) and coefficient of allometry (AC). Seed priming with gibberelic acid (GA) and salicylic acid (SA) (p≤ 0.01) increased germination characteristics as the compared to the unprimed. Seed priming with concentrations 25, 50 ppm of GA for 15 h at 10 °C and 25 ppm of SA for 12 h at 10 °C, may be considered as optimal treatment for priming of Secale montanum seeds in drought stress conditions. Also priming increased catalase (CAT) and ascorbate peroxidase (APX) as compared to the unprimed.
Authors and Affiliations
O. Ansari, M. Azadi, F. Sharif-Zadeh, E. Younesi
Keywords
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