Effect of iron overload on electrophysiology of slow reaction autorhythmic cells of left ventricular outflow tract in guinea pigs
Journal Title: Asian Pacific Journal of Tropical Medicine - Year 2018, Vol 11, Issue 2
Abstract
Objective: To investigate the electrophysiology effects and mechanism of iron overload on the slow response autorhythmic cells in the left ventricular outflow tract of guinea pigs. Methods: Standard microelectrode cell recording techniques were adopted to observe the electrophysiological effects of different concentrations of Fe2+ (100 μmol/L, 200 μmol/L) on the left ventricular outflow tract autorhythmic cells. Heart tissues were perfused with FeSO4 (200 μmol/L) combing with CaCl2 (4.2 mmol/L), Verapamil, (1 μmol/L), and nickel chloride (200μmol/L) respectively to observe the influences of these contents on electrophysiology of FeSO4 (200μmol/L) on the left ventricular outflow tract autorhythmic cells. Results: Fe2+ at both 100 μmol/L and 200 μmol/L could change the electrophysiological parameters of the slow response autorhythmic cells of the left ventricular outflow tract in a concentrationdependent manner resulting into decrease in Vmax, APA and MDP, slower RPF and VDD, and prolonged APD50 and APD90 (P all <0.05). Besides, perfusion of increased Ca2+ concentration could partially offset the electrophysiological effects of Fe2+ (200 μmol/L). The L-type calcium channel (LTCC) blocker Verapamil (1 μmol/L) could block the electrophysiological effects of Fe2+ (200 μmol/L). But the T-type calcium channel (TTCC) blocker nickel chloride (NiCl2, 200 μmol/L) could not block the electrophysiological effects of Fe2+ (200 μmol/L). Conclusions: Fe2+ can directly change the electrophysiological characteristics of the slow response autorhythmic cells of the left ventricular outflow tract probably through the L-type calcium channel.
Authors and Affiliations
Ling Fan, Li-Feng Chen
Keywords
Related Articles
- EP ID EP268126
- DOI 10.4103/1995-7645.225024
- Views 76
- Downloads 0
An overview on cardioprotective and anti-diabetic effects of thymoquinone
Thymoquinone (TQ), one of the active components of Nigella sativa exhibited to have many biological effects. Several beneficial effects of TQ such as its antidiabetic, antioxidant, anticancer, hypolipidemic, and anti-inf...
Laboratory diagnosis of schistosomiasis mansoni: Current status and future trends
Schistosomiasis is a neglected tropical disease that affects about 290 million patients worldwide. Children aged between 5 and 14 years represent 45.8% of the affected patients, in addition, schistosomiasis has been repo...
Diversity of livestock associated methicillin-resistant Staphylococcus aureus
Objective: To evaluate the diversity and molecular characteristics of livestock associated methicillin-resistant Staphylococcus aureus in livestock animals, food of animal origin and the environment in the Czech Republic...
Clinical and environmental isolates of Burkholderia pseudomallei from Brazil: Genotyping and detection of virulence gene
Objective To evaluate the genetic diversity of clinical and environmental isolates of Burkholderia pseudomallei (B. pseudomallei) recovered in Ceará, Brazil, and screen these isolates for the presence of type three secr...
Clinical study inpatient-reported outcomes after binocular implantation of aspheric intraocular lens of different negative spherical aberrations
Objective: To compare patient-reported outcomes after implantation of the ZA9003 intraocular lens (IOLs), or the MCX11 ASP IOLs or the spherical IOLs (HQ-201HEP). Methods: Prospective nonrandomized controlled trial was u...