Effect of kaempferol on the production and gene expression of monocyte chemoattractant protein-1 in J774.2 macrophages.
Journal Title: Pharmacological Reports - Year 2005, Vol 57, Issue 1
Abstract
Monocyte chemoattractant protein-1 (MCP-1) is produced by activated macrophages, and is involved in pathogenesis of cardiovascular and neurodegenerative disorders. There is a need to develop drugs that inhibit excessive infiltration of monocytes and lymphocytes to the arterial wall and central nervous system. The aim of this study was to evaluate the effect of kaempferol on the (MCP-1) gene expression and MCP-1 protein release by J774.2 macrophage cultures in vitro. Kaempferol given both before and after lipopolysaccharide (LPS) administration reduced secretion of MCP-1. Kaempferol administered before LPS stimulation significantly decreased the number of copies of MCP-1 mRNA. The results suggest that kaempferol inhibits MCP-1 production at the transcriptional level, and that this is an additional anti-inflammatory mechanism of action of this flavonoid.
Authors and Affiliations
Jan Kowalski, Arkadiusz Samojedny, Monika Paul, Grażyna Pietsz, Tadeusz Wilczok
Keywords
Related Articles
- EP ID EP144288
- DOI -
- Views 90
- Downloads 0
Age-dependent stimulatory effect of desipramine and fluoxetine pretreatment on metastasis formation by B16F10 melanoma in male C57BL/6 mice.
Although recent data may provide theoretical support for the preventive use of antidepressants in cancer patients, so far no study has demonstrated the clinical benefits of such strategies in the general population of ca...
Effects of two isomers of DDT and their metabolite DDE on CYP1A1 and AhR function in human placental cells.
The aim of this study was to investigate the actions of two isomers of DDT (p,p'-DDT, o,p'-DDT) and DDE (p,p'-DDE, o,p'-DDE) on the human placenta. We studied the effects of DDT and its metabolite DDE on CYP1A1 activity...
Effect of CYP2C9 and VKORC1 genetic variations on warfarin dose requirements in Indian patients.
Background: Warfarin, an oral anticoagulant is used in patients who are at increased risk of developing blood clots. The management of warfarin therapy is challenging because it shows large inter and intra individual var...
Treatment of inflammatory bowel disease (IBD).
Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract, which includes Crohn's disease (CD) and ulcerative colitis (UC). These diseases have become important health problems. Med...
Effect of pregnancy and tobacco smoke on the antioxidant activity of rutin in an animal model.
Tobacco smoke is a source of free radicals and causes oxidative stress in smokers' tissues. The aim of the current study was to evaluate the effect of rutin on the total antioxidant status (TAS) in pregnant and non-pregn...