Effect of low molecular weight heparin on autotransplantation efficacy of ovarian tissues in rats
Journal Title: Clinical and Experimental Obstetrics & Gynecology - Year 2019, Vol 46, Issue 4
Abstract
Background: One of the most important problems of ovarian transplantation is the significant reduction in the number of primordial follicles during the short period after transplantation due to post-transplantation ischemia. The aim of the work was to evaluate the effect of enoxaparin application on follicular survival after cryopreserved and fresh ovary transplantation in rats. Materials and Methods: The study used female Sprague Dawley rats (n=56). The animals were ovariectomized and the healing of fresh and cryopreserved ovarian tissue was evaluated. Estrogen blood level, percentage of ovarian live tissue, and angiogenesis were evaluated 16 days after transplantation of fresh ovary and 7, 14, and 28 days after transplantation of cryopreserved ovarian tissue. The animals in the experimental groups were treated after transplantation by enoxaparin subcutaneously in doses of 200 IU/kg per day. Results: A statistically significant higher (p < 0.05) percentage of live tissue was recorded in the treated goup (14.5±6.5 vs. 20.6±5.7% in control vs. treated groups) 7 days after transplantation of cryopreserved ovarian tissue, and a significantly more intensive (p<0.005) neoangiogenesis was recorded in the treated group (12.7±1.9 capillaries in 250 μm 2 ) in comparison with the control group (7.4±2.1 capillaries in 250 μm 2 ) 28 days after transplantation of cryopreserved ovarian tissue. Conclusions: Administration of enoxaparin after transplantation of cryopreserved ovarian tissue may temporarily improve tissue survival. The use of enoxaparin does not adversely affect neoangiogenesis around the transplanted ovary. The positive effect of low molecular weight heparin application on neoangiogenesis was demonstrated four weeks after transplantation.
Authors and Affiliations
T. Toporcer, I. Uhrinová, S. F. Iannaccone, A. Grendelová, F. Sabol, J. Belák, S. Toporcerová
Keywords
Related Articles
- EP ID EP672753
- DOI 10.12891/ceog4544.2019
- Views 52
- Downloads 0
Anti-N-methyl-D-aspartate receptor encephalitis associated with a tiny ovarian teratoma diagnosed by exploratory laparoscopy: report of two cases
Anti-N-methyl-D-aspartate (NMDA) receptor encephalitis is the most common form of paraneoplastic encephalitis that is associated with teratomas. Because removal of the tumor leads to better clinical outcomes, determining...
The effect of VEGF and Ang-1 on cryopreserved human ovarian grafts in severe combined immunodeficient mice
This study investigates the independent and combined effect of vascular epithelial growth factor (VEGF) and angiopoietin 1 (Ang- 1) on the follicle survival and vasculogenesis of cryopreserved human ovarian grafts in sev...
Femur length in normal fetuses and the correlation between crown-rump length and gestational age at 11–14 weeks in a Chinese Han population: a preliminary study
Objective: To establish normative data for the distribution of fetal femur length (FL) with increasing crown-rump length (CRL) and gestational age (GA) at 11–14 weeks, and to construct equations of fetal FL among normal...
Prevalence and associated risk factors of gestational diabetes mellitus in a tertiary hospital in Iran
Purpose of investigation: To evaluate the risk factors of gestational diabetes mellitus (GDM) to decrease maternal and neonatal complications. Materials and Methods: This cross- sectional study was performed on parturien...
A novel treatment of severe dysmenorrhea due to adenomyosis: a case series of 3 infertile patients treated with dopamine agonist and in vitro fertilization-embryo transfer resulting in successful pregnancy
Few therapies for adenomyosis are available for infertile patients with reduced ovarian reserve. Previous studies investigating dopamine agonists on endometriosis have reported a reduction of peritoneal endometriosis and...