Effect of low molecular weight heparin on autotransplantation efficacy of ovarian tissues in rats
Journal Title: Clinical and Experimental Obstetrics & Gynecology - Year 2019, Vol 46, Issue 4
Abstract
Background: One of the most important problems of ovarian transplantation is the significant reduction in the number of primordial follicles during the short period after transplantation due to post-transplantation ischemia. The aim of the work was to evaluate the effect of enoxaparin application on follicular survival after cryopreserved and fresh ovary transplantation in rats. Materials and Methods: The study used female Sprague Dawley rats (n=56). The animals were ovariectomized and the healing of fresh and cryopreserved ovarian tissue was evaluated. Estrogen blood level, percentage of ovarian live tissue, and angiogenesis were evaluated 16 days after transplantation of fresh ovary and 7, 14, and 28 days after transplantation of cryopreserved ovarian tissue. The animals in the experimental groups were treated after transplantation by enoxaparin subcutaneously in doses of 200 IU/kg per day. Results: A statistically significant higher (p < 0.05) percentage of live tissue was recorded in the treated goup (14.5±6.5 vs. 20.6±5.7% in control vs. treated groups) 7 days after transplantation of cryopreserved ovarian tissue, and a significantly more intensive (p<0.005) neoangiogenesis was recorded in the treated group (12.7±1.9 capillaries in 250 μm 2 ) in comparison with the control group (7.4±2.1 capillaries in 250 μm 2 ) 28 days after transplantation of cryopreserved ovarian tissue. Conclusions: Administration of enoxaparin after transplantation of cryopreserved ovarian tissue may temporarily improve tissue survival. The use of enoxaparin does not adversely affect neoangiogenesis around the transplanted ovary. The positive effect of low molecular weight heparin application on neoangiogenesis was demonstrated four weeks after transplantation.
Authors and Affiliations
T. Toporcer, I. Uhrinová, S. F. Iannaccone, A. Grendelová, F. Sabol, J. Belák, S. Toporcerová
Keywords
Related Articles
- EP ID EP672753
- DOI 10.12891/ceog4544.2019
- Views 47
- Downloads 0
Intestinal volvulus without malrotation in a dichorionic diamniotic twin: case report and review of literature
Volvulus is a condition in which the small bowel and proximal colon twist around the superior mesenteric artery, and a surgical emergency associated with high intrauterine morbidity and mortality. Intestinal volvulus can...
Vaginal microbiota composition as a diagnostic tool for bacterial vaginosis in pregnant Korean women
Objectives: To analyze the relative abundance of five Lactobacillus species, Gardnerella vaginalis, and Atopobium vaginae in the vaginal microbiota of pregnant Korean women to establish diagnostic criteria for bacterial...
Superficial cervical scar endometriosis following cone biopsy
Background: Cervical cone biopsy is a common simple surgical procedure for cervical intraepithelial neoplasia and cervical microinvasive carcinoma, with post-surgical bleeding being a common complication. Case Report: A...
Partners sexual dysfunctions as problems in gynecological praxis - a case of men libido disorder associated with rare endocrine disruption (Addison’s disease)
The authors present a case that clearly shows how the essential the element of gynecological anamnesis is the issue of sexual life and partner problems.The first author is a gynecologist and sexologist. A 58-year-old wom...
Increased Th17 and reduced Treg cells in patients with unexplained recurrent spontaneous abortion
Immune imbalance of T cells in the maternal-fetal interface is reported to be associated with unexplained recurrent spontaneous abortion (URSA), such as Th17/Tregs/Th1/Th2 cell. The authors’ previous finding demonstrated...