Effect of melatonin and/or cysteamine on development and vitrification of buffalo embryos
Journal Title: Asian Pacific Journal of Reproduction - Year 2017, Vol 6, Issue 4
Abstract
Objective: To assess the effects of melatonin and/or cysteamineon in vitro maturation, culturing and post-warming of buffalo embryos. Methods: Buffalo oocytes were classified into control, cysteamine (50 μM), melatonin (10 ng/mL) and cysteamine (50 μM) + melatonin (10 ng/mL) treatment groups. In experiment 1, previous treatments were added during in vitro maturation and culturing of buffalo oocytes. Results: Cleavage and blastocyst rates were significantly (P<0.05) increased in melatonin treated group (70.5±0.9 and 12.8±1.0, respectively). However this effect was potentiated when combined with cysteamine (74.0±1.7 and 14.8±1.7, respectively). In experiment 2, the treatements were added in maturtaion, culturing as well as post-warming culture media. Embryos at 7 d were vitrified.Viability assessement directly after warming showed significant increase (P<0.05) in cysteamine, melatonin and their combination groups (76.8±2.8, 80.0±2.1 and 83.3±1.7, respectively) than control (65.8±2.4); but the viability after 24 h post-warming was the best in cysteamine + melatonin combination group (61.4±2.1). Conculsions: Enriching maturation, culturing and post-warming media of buffalo oocytes and embryos with melatonin and/or cysteamine have significantly beneficial effects on oocyte developmental competence as well as embryos vitrification procedure outcomes which in turn resulting in enhancement of commercial buffalo embryo production.
Authors and Affiliations
Mohamed MM El-Sokary, Mohamed El-Raey, Karima GhM Mahmoud, Mahmoud EA Abou El-Roos, Gamal MS Sosa
Keywords
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- EP ID EP240845
- DOI 10.12980/apjr.6.20170406
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