Effect of Methyl-B-Cyclodextrin (MBCD) on In Vitro Capacitation of Buffalo Frozen/Thawed Sperm
Journal Title: Journal of Buffalo Science - Year 2014, Vol 3, Issue 1
Abstract
The aim of this study was to determine the effect of Methyl-B-Cyclodextrin (MBCD) on capacitation of buffalo sperm. Frozen/thawed semen was incubated in the absence of capacitating agents (negative control), in the presence of 10 g/ml heparin (positive control) and of 1, 2, 4 and 8 mg/ml MBCD for 2 and 4h. At each incubation time, sperm motility was evaluated by phase contrast microscopy. Capacitation was assessed by the sperm ability to undergo acrosome reaction after lysophosphatidylcholine treatment, evaluated with viability by Trypan blue-Giemsa. After 2 h capacitation increased (P<0.01) in MBCD groups (39.2±1.4, 44.5±3.3, 56.7±1.5 and 62.5±3.8, with 1, 2, 4 and 8 mg/ml MBCD, respectively) compared to the negative and positive controls (27.5±1.0 and 28.0±0.8, respectively). Likewise, after 4 h the percentage of live capacitated sperm was higher at increasing concentration of MBCD (31.0±0.7, 34.5±1.7, 42.0±1.9, 49.2±2.8, 62.3±1.5 and 70.8±1.7 in negative control, positive control and with 1, 2, 4 and 8 mg/ml MBCD, respectively; P<0.01). After 2 h sperm motility was lower (P<0.01) in 4 and 8 mg/ml MBCD groups (43.3±2.1 and 25.0±3.2, respectively) than in negative control, positive control, 1 and 2 mg/ml MBCD groups (55.0±1.8, 48.3±2.8, 61.7±2.8, 56.7±1.1, respectively). After 4 h the lowest sperm motility was observed with higher MBCD concentrations (40.0±0.0, 46.7±4.2, 51.7±4.6, 50.0±0.0, 40.0±3.7 and 6.7±1.1, in negative control, positive control, 1, 2, 4 and 8 mg/ml MBCD, respectively; P<0.01). In conclusion, MBCD improved sperm capacitation in a dose-dependent manner while decreasing the sperm motility at higher concentrations.
Authors and Affiliations
A. R. Elkhawagah, V. Longobardi, B. Gasparrini, G. A. Sosa, A. Salzano, M. E. A. Aboul-roos, A. E. Abd El-Gaffar and L. Zicarelli
Keywords
Related Articles
- EP ID EP33576
- DOI -
- Views 317
- Downloads 0
Characterization of Seasonal Variations in Responsiveness of Pituitary Gland to Different Doses of Gonadotropin Releasing Hormone in Buffalo Cows
In tropical countries such as India, it has been observed that a number of buffalo cows experience seasonal anestrous during summer months. This might be due to seasonal changes in responsiveness of pituitary gland to...
Evaluation of Genetic Merit of Buffaloes by Direct Sequencing
Sequencing the genome of the buffaloes enables scientists to more accurately identify the genetic markers that are responsible for economically important traits such as milk yield, milk fat and protein. Producers can us...
Serum Profile of Thyroid Hormones from Birth to Puberty in Buffalo Calves and Heifers
Growth is outcome of interactions among several factors, where thyroid hormones play a key role in coordination of these factors and the information of thyroid hormones in relation to growth in buffalo calves and heifer...
The Pregnancy Diagnosis in Buffalo Species: Laboratory Methods
Pregnancy diagnosis plays an important role in the reproduction management of ruminants since embryonic mortality has a substantial impact on the fertility of a herd. Most of the embryonic losses occur during the first...
Mesopotamian Buffaloes (The Origin)
The aim of this study to throw light on Iraqi Buffalo origin, comparing between past archeological theories based on ivory materials and cylindrical seals findings in Mesopotamia and recent molecular biological studies...