Effect of Molecular Weight on Electro Spun Pcl Based Composite Fibrous Mats
Journal Title: Biomedical Journal of Scientific & Technical Research (BJSTR) - Year 2017, Vol 1, Issue 4
Abstract
Controlling the biodegradation of scaffolds to tune with that of the growing tissue is crucial for tissue regeneration. Although molecular weight of a polymer is known to influence its degradation, such studies have not been reported for electrospun Polycaprolactone (PCL) based composite scaffolds. In the present study, two low molecular weight PCL, 10 and 45 kDa PCL (10PCL and 45PCL) have been analyzed for their electrospun ability and biodegradation in comparison with 80 kDa PCL (80PCL) in forming pure polymeric and hydroxyapatite composite nanofibrous mats. The 45PCL and 80PCL were electro spin able but the 10PCL resulted in electro spraying of particles. However, 45PCL needed a higher concentration (20 wt.%) of polymer than the 80PCL (8 wt.%) in order to electrospin bead free uniform fibres. The fibres obtained were in the diameter range of 310±50 nm and 400±100 nm for the 45PCL and 45PCL/HA composite mats respectively. The Hydroxyapatite (HA) incorporation in the composites was confirmed through X-ray diffraction and spectroscopic methods. The 45PCL/HA composite degrades at a relatively faster rate than the 80PCL/HA composite. The electrospun mats were also found to be non cytotoxic (Live/Dead assay), commendable cellular metabolic activity (MTT assay) and good proliferation of osteoblast like cells (HOS) as evidenced through fluorescent and scanning electron microscopy. This work demonstrated the feasibility of fabricating PCL based electrospun composite scaffolds having controlled degradation rates.
Authors and Affiliations
TS Sampath Kumar, Pooja Vardhini Natesan, V Yogeshwar Chakrapani
Keywords
Related Articles
- EP ID EP571016
- DOI 10.26717/BJSTR.2017.01.000379
- Views 148
- Downloads 0
Postural Difference in Expiratory Rate among Female Sanitary Workers and Its Relationship with Blood Pressure and Anthropometric Indices
Numerous studies have documented the adverse effects of occupational hazards on respiratory functions. The present study hypothesized that: Peak Expiratory Flow Rate (PEFR) of Nigerian premenopausal sanitary workers was...
Lingual Orthodontics: The Changing Trends
Lingual braces are one of the “invisible” treatment methods for correction of malaligned teeth for the most part, they look like conventional dental braces that have been mounted on the backside (lingual, tongue side) of...
The Effects of Pulmonary Arterial Hypertension on the Heart Work Dynamics: Gender Differences
Aim: The aim of this paper was to find out possible changes of heart work dynamics induced by pulmonary artery hypertension in male and female rats. Materials and methods: Experiments were performed on the Wistar rats of...
Assessment of Cognitive Impairment in Older Research Participants
In any clinical trial or observational study, a certain level of cognitive function is required in order to ensure participant safety and ability to comply with the study protocol. Participants should adequately understa...
A Case report: Importance of Molecular Allergy Diagnostics in Hen’s Egg Allergy
Background: Egg allergy is common in children. Most of the allergy outgrown with age. Oral food challenge (OFC) has been used in allergy diagnostics. Reference values within which 95% of the subjects with positive exposu...