Effect of Nicotine Exposure on Mandibular Development of CD-1 Mouse Fetuses: A Histological Study
Journal Title: Journal of Dental Health and Oral Research - Year 2024, Vol 5, Issue 2
Abstract
No Previous investigation examined the histological effects of nicotine on mandible development in mouse. Therefore, fetuses of pregnant CD-1 mice, exposed to 0.1% nicotine sulfate at a dose of 1.67 mg/kg body weight from the 6th to the 15th gestational day, were compared with controls (Saline-injected and untreated) fetuses. This to assess the histologic effects of nicotine on mandibular osteogenesis. Mothers were sacrificed on the 18th day of gestation. The 180 decapitated nicotine-treated heads as well as the 348 control heads were measured (height, width and circumference), embedded in paraffin, sectioned in the frontal plane, 5 µm thick and stained with H&E for light microscopic examination. Data analysis revealed the head dimensions were significantly reduced in nicotine treated heads when compared to those of controls. Histologic examination demonstrated 9.6% of fetuses of experimental heads presented cleft palate, whereas none of the controls had that anomaly. Furthermore, development of the mandibles, with or without clefts, appear retarded, bony elements were smaller and less dense in experimental fetuses when compared to those of control groups. It was concluded that nicotine has a detrimental effect on head size and mandibular osteogenesis of CD-1 albino mice.
Authors and Affiliations
Abdelhamied Y Saad, Leslie P Gartner
Keywords
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- EP ID EP740922
- DOI https://doi.org/10.46889/JDHOR.2024.5208
- Views 22
- Downloads 0
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