Effect of Nisin on Technological and Microbiological Characteristics of Stirred Yoghurt
Journal Title: Journal of Microbiology & Microbial Technology - Year 2016, Vol 1, Issue 1
Abstract
Purpose of this paper: Growing worldwide inclination towards healthful foods has compelled food processors to search for natural preservatives to enhance the shelf-life and extend the market reach. Limited shelf-life of cultured milk products arising from post-acidification could be enhanced with the application of bacteriocins such as nisin. Application of nisin in stirred yoghurt may retain its desirable properties during extended shelf-life. Design/Methodology/Approach: Stirred yoghurt containing nisin was obtained by culturing cow milk, heated at 95 °C / 5 min with 3% Yoghurt-YH-3 incubated at 42 ± 1 °C for 5 h and then stirred for incorporating 25 RU/ml nisin, followed by further 1 h incubation. Effect of incorporation of nisin on technological (titratable acidity, diacetyl and acetoin content, volatile acidity and extent of proteolysis and microbiological (total viable microbial contents, coliform count, yeast and mould, lipolytic organism and proteolytic organisms) and antibacterial characteristics of stirred yoghurt were assessed during 40 days of storage at 10 - 15 °C. Findings: Incorporation of nisin in yoghurt induced lower acid and volatile acid development, but a significantly higher proteolytic activity as well as diacetyl + acetoin production. Nisin exhibitedantagonism against viable population but ineffective to yeast,mould and proteolytic organisms in yoghurt Nisin containing yoghurt exhibited lower degree of antagonism against various pathogenic test organisms, which progressive increased during storage.
Authors and Affiliations
Sarkar S
Keywords
Related Articles
- EP ID EP246373
- DOI 10.13188/2474-4530.1000004
- Views 120
- Downloads 0
Single Cell Protein: A Resort to Global Protein Challenge and Waste Management
The increase in human population, death from starvation, civil unrest, malnutrition and related diseases in countries of the world call for serious remedy. Agriculture alone cannot meet with the high demands of protein r...
Nocardiosis: An Emerging Infectious Actinomycetic Disease of Humans and Animals
In recent past, many infectious diseases of diverse etiologies have emerged, which resulted into significant morbidity and mortality in humans as well as in a variety of animals. Among these diseases, nocardiosis is one,...
Biofuels in Croatia
European Commission has adopted a series of documents in order to achieve energy policy targets. One of them is Directive 2009/28/EC which promotes the use of renewable energy in transportation. The Directive sets target...
Removal of Mixed Azo Dyes by a Moderately Alkaliphilic Bacterial Strain Isolated from Textile Effluent Contaminated Site
The aim of the present study was to isolate an alkaliphilic bacterial strain from textile effluent contaminated site and to investigate the ability of the bacterial strain to decolorize the mixed dyes, Direct Brown MR an...
Preliminary Characterization of a NADPH Dependent Chromate Reductase from Trichoderma pseudokoningii
Intracellular chromate reductase enzyme extracted from a chromium tolerant strain Trichoderma pseudokoningii was partially purified by ultrafiltration and gel filtration chromatography. The purified fraction showed highe...