Effect of Orthosiphon stamineus Extract on HIF-1Α, Endothelin-1, and VEGFR-2 Gene Expression in NRK-52E Renal Tubular Cells Subjected to Glucotixicity
Journal Title: International Journal of Experimental Research and Review - Year 2024, Vol 37, Issue 1
Abstract
This study aimed to investigate the impact of Orthosiphon stamineus extract on gene expression in NRK-52E cells under conditions of glucotoxicity. Gene expression analysis using RT-PCR was conducted following exposure of cultured NRK-52E cells to glucotoxic conditions and various concentrations of Orthosiphon stamineus extract. The results revealed a dose-dependent decrease in HIF-1α, ET-1 and VEGFR-2 gene expression levels upon treatment with Orthosiphon stamineus extract. The diverse array of phytochemicals found within Orthosiphon demonstrates a significant impact on the biochemical pathways implicated in the pathogenesis of the disease. Additionally, molecular docking studies suggested the potential inhibition of Rho-kinase as a mechanistic explanation for this observed effect. These findings suggest that Orthosiphon stamineus extract may possess renoprotective properties against glucotoxicity-induced cellular damage. This study contributes to understanding potential therapeutic interventions for managing renal complications associated with conditions such as diabetes.
Authors and Affiliations
Devaprasad Markandeyan, Shiek S. S. J. Ahmed, Palani Perumal, Arulvasu Chinnasamy, Bhavatarini Govindaraj, Niranjni Sekar, Sudhan Mookandi, Priyadarshini Shanmugam, Janakiraman Velayudam, Deepak Rajasekar Padmanaban, Perumal Jayaraman
Keywords
Related Articles
- EP ID EP733359
- DOI 10.52756/ijerr.2024.v37spl.015
- Views 46
- Downloads 1
QbD-Driven Development and Validation of a Bioanalytical LC–MS Method for Quantification of Paliperidone in Human Plasma
This paper discusses how a Quality by Design (QbD) strategy was used to develop and test an HPLC-MS bioanalytical method for detecting plasma Paliperidone concentration. A C18 column and an isocratic mobile phase of orga...
Variation in the stored grain pest Sitotroga cerealella (Olivier) infestation at low and high moisture storage conditions among some indigenous rice genotypes of West Bengal
The food security programme is negatively impacted by postharvest losses (PHLs). In terms of providing food for a nation, proper grain postharvest (PHV) storage is more crucial than intense and widespread farming. Reduci...
User Interface Bug Classification Model Using ML and NLP Techniques: A Comparative Performance Analysis of ML Models
Analyzing user interface (UI) bugs is an important step taken by testers and developers to assess the usability of the software product. UI bug classification helps in understanding the nature and cause of software failu...
Idol immersion in Ichhamati river and its impact on water quality parameters
A preliminary study was undertaken in Ichhamati river, Bongaon, N-24 Parganas, West Bengal to evaluate the impact of idol immersion after Durga puja on water quality parameters. Different important physico-chemical param...
Identifying and Ranking Critical Motivational Dimensions for the Choice of Wellness Tourism: An Analytic Hierarchy Process (AHP) Approach
This exploratory study aims to determine the elements that impact travellers' decisions to choose wellness tourism. Motivation criteria have been prioritized to determine the most influential reason tourists decide to en...