EFFECT OF PH AND TIME ON CALCIUM RELEASE FROM SOUND ENAMEL: AN ATOMIC SPECTROMETRIC ANALYSIS
Journal Title: International Research Journal of Pharmacy (IRJP) - Year 2013, Vol 4, Issue 6
Abstract
Dental Caries is a disease of great concern which affects people of all races in all places, the exact etiology of caries though unknown; it is well established that the release of calcium and phosphate from hydroxyapatite crystal is due to decreased pH of saliva. However the relationship between calcium release, pH and time is not well documented. The present study was designed to assess the calcium release from intact enamel when subjected to different pH at different time intervals. The enamel samples were subjected to acid challenge using Acetate buffer of pH of 3.5, 4, 4.5, 5, 5.5, 6 and 6.5 and buffer solution were analyzed for calcium release at specific time intervals for each pH using atomic absorption spectrometry. The results show that the rate of calcium release significantly increased from pH 5.0 to 3.5, however the calcium release was very little in pH 5.5 to 6.5. From the observations made from this study we can conclude that sound enamel is resistant to acid attack between pH of 5 to 5.5 and Time plays an important role in the reaction of enamel to acid.
Authors and Affiliations
Shishir Shetty , Mithra Hegde , Suchetha Kumari , Smitha Shetty , Mahesh Bekal , Thimmaiah
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