Effect of phytohormones on shoot apex and leaf explants of Withania somnifera (Ashwagandha)
Journal Title: Journal of Applied and Natural Science - Year 2016, Vol 8, Issue 1
Abstract
An efficient protocol for callus induction of Withania somnifera through in vitro culture of shoot apex and leaf explant was standardized. Of the various combinations of phytohormones evaluated, MS media supplemented with 6-furfuryl aminopurine (KIN) 0.5 mg/l + 2,4-dichlorophenoxy acetic acid (2, 4-D) 2.0 mg/l was found to be best for mean callus induction (86%) in leaf explants after 6 weeks of culture and in case of shoot apex expant the best response and growth of callusing was observed on MS medium supplemented with 2,4-D 1.0 mg/l + BAP 2.0 mg/l (77%).The response of callus growth increases gradually with the reductions in concentration of KIN in culture medium of both the explants. This protocol might be used in further research for mass propagation of W. somnifera via indirect regeneration methods.
Authors and Affiliations
Archana Rani, M. Kumar, Sanjeev Kumar
Keywords
Related Articles
- EP ID EP284473
- DOI -
- Views 85
- Downloads 0
Sclerotinia rot of rapeseed mustard: A comprehensive review
Indian mustard [Brassica juncea (L.) Czern & Coss.] is one of the major oilseed crops cultivated in India and around the world. It is extensively grown traditionally as a pure crop as well as intercrop (mixed crop) in ma...
Studies on influence of genotypic diversity on yield, quality and incidence of white fly and yellow mite in Capsicum annuum L.
A field experiment was conducted with fifty one chilli (Capsicum annuum L.) genotypes to study the growth, yield, quality characters, white fly and yellow mite incidence during Rabi season of 2007-08 and 2008-09. The dat...
Effect of agro-input management practices on yield of linseed (Linum usitatissimum L.) under vertisols of Chhattisgarh, India
A field experiment was conducted to study the effect of agro-input management practices on yield of linseed (Linum usitatissimum L.) at the Instructional cum Research Farm, Indira Gandhi Krishi Vishwavidyalaya, Raipur, (...
Isolation and characterization of Fusarium oxysporum, a wilt causing fungus, for its pathogenic and non-pathogenic nature in tomato (Solanum lycopersicum)
Collection of soil and plant samples from nine different geographical locations in Uttar Pradesh state of India was made. Composite soil was analyzed for its nutrient status. A total of sixty isolates of Fusarium species...
Biochemical analysis of Psophocarpus tetrgonolobus L. (Winged bean) and its role on restoration of degraded land of Raniganj and Barjora coalmine areas of West Bengal, India
To meet the growing demands of energy, continuous supply of coal is necessary because coal is the primary source of energy. About 70% of coal mining is being done by open cast methods which is most destructive activity a...