EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO CALLUS INDUCTION OF SHOOT EXPLANT MANGOSTEEN (GARCINIA MANGOSTANA L.)
Journal Title: International Journal of Advanced Research (IJAR) - Year 2018, Vol 6, Issue 11
Abstract
Introduction: The productions of generatively mangosteen still has problem because the seeds of mangosteen have recalcitrant characteristic, which means the seeds must be planted immediately, since the seeds does not have dormancy period. Aim: 1) to determine Effect of Plant Growth Regulators on in vitro Callus Induction of mangosteen (Garcinia mangostana L.), 2) to know the best combination and composition of the media to in vitro callus induction of mangosteen (Garcinia mangostana L.). Method: Researcher used complete randomized design (CRD) factorial which is consisting of 12 treatments with 3 times repetitions. The research procedures were: tool sterilization, explant sterilization, media making, explants planting. Result: The fastest response of callus appearance is on sucrose (15; 30 gr / L) and NAA (1,5; 3 ppm) treatment which is producing callus with crumb texture and color is identical to green and white. The callus has 0,53a cm in height, 1,56a cm2 in width, and 0,40a gr in weight. The percentage of callus formation is 100% and the explant response is growing all over the surface (sucrose 15 gr / L + NAA 1,5; 3 ppm). Conclusion: The plant growth regulators have effect on in vitro callus induction of shoot explant mangosteen (Garcinia mangostana L.). The best media combination and composition on in vitro callus induction of shoot explant mangosteen (Garcinia mangostana L.) were sucrose (15; 30 gr / L) and NAA (1,5; 3 ppm).
Authors and Affiliations
Ayu Yusna, Fauziyah Harahap, Syahmi Edi.
Keywords
Related Articles
- EP ID EP423091
- DOI 10.21474/IJAR01/7978
- Views 74
- Downloads 0
URBAN ENVIRONMENTAL CONSERVATION MEASURES IN KODAIKANAL REGION TAMIL NADU.
It is clear that the world is becoming more and more urban. It has been predicted that by the year 2050, 70% of the human population on Earth will live in cities?. There are many environmental problems that are associate...
COMPUTATIONAL ANALYSIS ON GENE EXPRESSION PATTERN: A SURVEY.
A gene is a part of Deoxyribonucleic acid (DNA), which contains all the information required to analysis the defects and genetic problems that evolves in an organism. A gene is also the element of information that is tra...
SWOT ANALYSIS OF MIXED OWNERSHIP REFORM IN POWER GRID ENTERPRISES: A CASE STUDY IN WESTERN INNER MONGOLIA.
Mixed ownership reform and reform of electric power system have brought new opportunities and challenges to the development of China\'s power grid enterprises. Taking Inner Mongolia West Power Grid as an example, this pa...
A SURVEY ON THE DIVERSITY OF MITES INHABITING THE ORNAMENTAL AND FRUIT PLANTS IN SOUTH BENGAL, INDIA.
Mites are akin to spiders and most of them are inhabitant of plants. Phytophagous mites cause depletion in quality and quantity of ornamental and floricultural as well as fruit plants and thus causing substantial economi...
A STUDY OF THE POLITICAL CHANGES IN UNDIVIDED BENGAL AND WEST BENGAL (1937 - 1967).
International Journal of Advanced Research (IJAR)