EFFECT OF POLYSORBATE-80 CONCENTRATION ON G-CSF FORMULATION USING LIQUID CHROMATOGRAPHY
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2014, Vol 6, Issue 9
Abstract
Objective: The aim of the study was to find the dual effects of polysorbate-80 concentration on oxidation and aggregation in the formulation of granulocyte colony stimulating factor.Methods: The effect of polysorbate-80 at different concentration was monitored and analyzed using high performance liquid chromatography. Oxidative degradation and aggregate generation was studied using reverse phase and size exclusion chromatography method respectively.Results: With increase in concentration of polysorbate-80 the amount of oxidation as well as aggregate formation increases in a concentration dependent manner. The aggregates present at higher concentration of polysorbate-80 formulation are not found with low concentration or without polysorbate-80. This result shows that higher concentration of Tween-80 force the formation of oligomers and leads to increased level of oxidation of granulocyte colony stimulating factor.Conclusion: The dual effect of aggregation and oxidation of polysorbate-80 on the recombinant granulocyte colony stimulating factor indicate that during formulation development studies it is crucial to evaluate the amount of polysorbate-80 to be used in the formulation.Â
Authors and Affiliations
Amand Bhaskar, Kunal Mukhopadhyay, Manish Kumar
Keywords
Related Articles
- EP ID EP579583
- DOI -
- Views 98
- Downloads 0
FORMULATION DEVELOPMENT AND EVALUATION OF ORALLY DISINTEGRATING TABLET OF CHLORPHENERAMINE MALEATE BY SUBLIMATION TECHNIQUE
Objective: Chlorpheneramine maleate is a first-generation antihistamine drug used in the treatment of allergic conditions like rhinitis, urticaria, and cough cold, etc. In present work, the challenge has been made to dev...
DESIGN AND EVALUATION OF MODIFIED CHITOSAN BASED IN SITU GEL FOR OCULAR DRUG DELIVERY
Objective: The object of the present study was to formulate and evaluate in-situ gel of modified chitosan by using temperature triggered method to improve bioavailability.Methods: Modified chitosan-based moxifloxacin HCl...
ANTIMICROBIAL PROPERTIES OF LEPTADENIA HASTATA (PERS) DECNE LEAVES EXTRACT
Objective: This study was carried out with an objective to investigate the antibacterial potential of five leaf extract of Leptadenia hastata plant on some bacteria.Methods: Leptadenia hastata extracts were evaluated for...
SYNTHESIS AND BIOLOGICAL EVALUATION OF NOVEL COUMARIN DERIVATIVES AS POTENTIAL ANTIMICROBIAL AGENTS
Objective: Synthesize new series of 7-hydroxy-4-methylcoumarin and 7-alkoxy-4-methylcoumarin derivatives featuring thiosemicarbazone or thiazolidin-4-one moieties and to evaluate their antimicrobial activity against two...
ESBL, MBL AND AMP C-β LACTAMASES PRODUCED BY SUPERBUGS: AN EMERGING THREAT TO CLINICAL THERAPEUTICS
Objectives: The present study was undertaken to determine the prevalence of multi drug resistant (MDR) and multiple β-lactamase producing Pseudomonas aeruginosa isolates in lower respiratory tract infection (LRTI) patie...