Effect of Portland cement on osteogenic and dentinogenic differentiation of stem cells from apical papilla (An in vitro study)
Journal Title: Scholars Journal of Dental Sciences - Year 2016, Vol 3, Issue 8
Abstract
Abstract: Advancement of regenerative treatments using stem cells, materials used in regenerative treatment could have be have the ability to stimulate hard tissue formation and positive effect on osteogenesis and dentinogenesis differentiation of stem cells. Stem cells from apical papilla (SCAPs) are one of the mesenchymal stem cells derived from the embryonic neural crest, with greater odonto/osteogenic capacity and outstanding dentinogenesis. Therefore in this study we evaluated the effect of Portland cement and on odontogenic and osteogenic differentiation of this cells. Multiple colony-derived stem cells were isolated from the apical papillae, and the effects of material on the differentiation of SCAPs were investigated in vitro by using ALP activity measure, alizarin red staining and Real Time PCR. Alizarin red and alkaline phosphatase staining showed mineralization in Portland group. The results of the evaluation of gene expression showed that the majority of the studied genes associated with the differentiation of osteogenic and odontogenic in Portland group increased significantly compared to the control group. (P < 0.001). Portland cement have the ability to induce mineralization process and stimulate osteogenic and odontogenic differentiation. Keywords: Stem cells from apical papilla, Regenerative treatments, Portland cement
Authors and Affiliations
Eshagh Ali Saberi, Narges farhad mollashahi, Sahar Soltani, Arezoo hooshmandi
Keywords
Related Articles
- EP ID EP373748
- DOI -
- Views 82
- Downloads 0
The Biologic Width- Importance and Management of Its Violation: A Review
Abstract: It is important to know the relationship between periodontal condition and prosthodontic restoration to ensure esthetics, function and comfort to patient. Biologic width plays a key factor in making the periodo...
Role of Herbals in Endodontics
Medical plants have a long history of use and their use is widespread in over world countries. According to the report of the World Health Organization 80% of the words population rely mainly on traditional therapies whi...
Dentists' Awareness about Management of Medical Emergencies in Dental Offices Birjand-2014
Abstract: Most of medical emergencies which take place in dental offices could be life-threatening. In this regard, the knowledge about these medical emergencies is of great importance and all of the dental staff should...
Facial Attractiveness: An Orthodontic Prospective
Abstract: As the saying “beauty is the ease of the beholding” there are various studies which have shown that people prefer attractive faces over unattractive faces. This article reviews about the facial attractiveness w...
Oral soft tissue Metastases of distant cancers
Abstract: The oral cavity is not a preferred site for metastasis of distant cancers, accounting for only 1% of all oral cancers. Almost all types of cancers can spread to the oral cavity, and usually are late presentatio...