Effect of Process Parameters on Dextran Production by Weissella Confusa
Journal Title: International Journal for Research in Applied Science and Engineering Technology (IJRASET) - Year 2015, Vol 3, Issue 11
Abstract
Dextran a bacterial polysaccharide and a polymer of glucose is produced by different microorganisms like Leuconostoc mesenteroides, Lactobacillus sps, Streptococcus mutants Weissella confusa etc. It has a wide range of applications in the food, pharmaceuticals and other industries. Dextran and its derivatives like iron dextran, clinical dextran, food grade dextran are rapidly emerging as new and industrially significant products. In this study, attempts were made to investigate the influence of various parameters such as pH, temperature, incubation period, agitation speed, aeration and inoculum size for dextran production by Weissella confusa in 4% sucrose medium. The optimum pH, temperature, incubation period, agitation, aeration and inoculum size for dextran production were found to be 6.5, 350C, 48 hours, 150 rpm, 1.5: 5 (medium to head space ratio), and 5% respectively. Dextran was recovered from broth by alcohol precipitation and this purified dextran was subjected to HPLC analysis and the results indicated that it was a low molecular weight dextran. Thus the studies indicates that low molecular weight dextran producer identified genetically as Weissella confusa can be used for commercial production of medically and industrially important dextran.
Authors and Affiliations
B. Srinivas, P. Naga Padma
Keywords
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