Effect of Process Variables on Survival of Bacteria in Probiotics Enriched Pomegranate Juice
Journal Title: Biotechnology Journal International - Year 2015, Vol 5, Issue 1
Abstract
Aims: The consumption of foods and beverages containing probiotic microorganisms is a growing, global consumer trend. In this research, production of probiotic pomegranate juice containing Lactobacillus plantarum and Lactobacillus delbrueckii was studied. Study Design: Plackett-Burman statistical design was used to evaluate the impact of eleven process variables on the viability of both probiotics. Impact of incorporation of grape juice, tomato juice and pomegranate peel extract as well as phenolic compounds and vitamins have been investigated. Place and Duration of Study: Department of Food Science and Technology, Varamin Branch, Islamic Azad University, Varamin, Tehran, Iran, between Sep 2012 and July 2013. Methodology: Pomegranate juices were inoculated with probiotic bacteria and their survival was evaluated every week by pure plate method. The effect of 11 variables (in two levels) on survival of bacteria by the statistical design of Plackett-Burman was evaluated. For this purpose, 12 treatments in triplicate by the Minitab (version = 11.0) software at significant levels α= .01 were analyzed. Results: The highest survival rate of L. plantarum (4.74 ×106 CFU/mL) and L. delbrueckii (4 ×106 CFU/mL) was obtained by 10% v/v inoculation of a 48 h inoculum culture in MRS broth medium to enriched pomegranate juice (10% v/v Grape juice, 5% v/v tomato juice, 0.1% v/v pomegranate peel extract and 2.0 g.L glucose) which was inoculated in anaerobic condition for 72 h at 37ºC and kept for 2 weeks at environment temperature. Sensory evaluation shows the probiotic juice was accepted by consumers with no significant difference in comparison to control in terms of taste, odour and overall acceptability (P>.05). Conclusion: The results of this study suggest that grape, tomato juices and pomegranate peel extract exert a protective effect on L. plantarum and L. delbrueckii viability under acidic condition of pomegranate juice and storage time, which was associated with the chemical composition of them. This study indicates that develop of probiotic pomegranate juices with acceptable viability and stability of the probiotic is possible.
Authors and Affiliations
Mina Khanbagi Dogahe, Kianoush Khosravi-Darani, Azadeh Tofighi, Mehrnaz Dadgar, Amir Mohammad Mortazavian
Keywords
Related Articles
- EP ID EP236906
- DOI 10.9734/BBJ/2015/12114
- Views 172
- Downloads 0
Lipase Production by Fungal Isolates Grown in Palm Oil Mill Effluent
Aims: The present work is aimed at determining the optimum conditions of pH, temperature and nitrogen concentration for lipase production by Candida rugosa and Geotrichum candidum in POME. Place and Duration of Study:...
Bio-bleaching of Anthocephalus cadamba Kraft Pulp through Direct Fungal Treatment by FEQP Sequence
Aims: The study aims at mitigating pulp kappa number before bleaching to minimize pollution load. Study Design: An experimental study. Methodology: The various parameters of direct fungal (Coprinellus disseminatus MLK01)...
Characterization of Spent Bleaching Earth and Its Utilization for Improving Manure-based Biogas Production
Aim of the Study: To utilize spent bleaching earth (SBE) to improve biogas production efficiency through co-digesting with manure and provide a practical way of disposing the SBE. Design of the Study: The SBE was collec...
Screening of Fungal Resources for the Production of Cellulases and Xylanases
Aims: The final screening of fungal isolates aimed at applications based tertiary screening i.e. deinking of mixed office waste paper and saccharification of pearl millet stover and cellulases from selected fungal isolat...
In-vitro Measurement of Glucose Concentration in Human Blood Plasma Mixed Intralipid Phantom Samples by Using Modulated Ultrasound and Infrared Light
Non-invasive blood glucose measurement is one of most innovative domain in Biomedical Engineering. Multiple methodologies have-been introduced over last few decades to fulfil the clinical requirement for non-invasive glu...