Effect of Purified Protein Fractions from Soybean on Tyrosinase Inhibition
Journal Title: Scholars Academic Journal of Biosciences - Year 2017, Vol 5, Issue 8
Abstract
Skin is an important barrier that protects the body from damage due to its direct contact with the outside environment. Skin has epidermal units that are responsible for melanin production and distribution, a process called melanogenesis that is regulated by various enzymes, including tyrosinase. Due to its central role in melanogenesis, tyrosinase is a key target for screening and discovery of new inhibitors. The aim of this study was to investigate the efficacy of purified protein fractions from soybean (Glycine max) on inhibiting tyrosinasein vitro. Protein was extracted by using isoprecipitation method, followed by protein purification using Fast Protein Liquid Chromatography (FPLC) with DEAE anion exchange system and gradient NaCl elution. Among 9 purified protein fractions, there were 2 protein fractions (10 µg/mL) with molecular weights of 42 and 46 kDa exerted significant inhibition up to 95% on tyrosinase activity in vitro. In conclusion, purified protein fraction from soybean demonstrated potential tyrosinase inhibitory effect, indicating their potential use on cosmeceutical application.
Authors and Affiliations
Sri Sustain De Hugo Sitanggang, Yanti ,, BibianaWidiyati Lay
Keywords
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