EFFECT OF PURSLANE (PORTULACA OLERACEA) ON RENAL ISCHEMIA/REPERFUSION INJURY IN RAT
Journal Title: International Journal of Pharmacy and Pharmaceutical Sciences - Year 2015, Vol 7, Issue 1
Abstract
Objective: Use of herbal medicines has increased in recent years and many researches show their values in the treatment and prevention of diseases. In several studies, antioxidant properties of purslane (PO) were demonstrated. The aim of the present study was to investigate the effect of ethanolic extract of PO (EEPO) on the renal function and antioxidant status after induction of ischemia reperfusion (I/R) injury in the rat kidney.Methods: 30 male Wistar rats were divided into 5 groups (n=6): sham operated+ vehicle (sham), sham operated+ EEPO 300mg/kg (control), I/R, I/R+ EEPO 150 and 300 mg/kg. Unilateral nephrectomy was performed in all animals, 20 days before the experiment. PO extract or vehicle was administered for 5 days, So that the last dose was administered a half hour before induction of the left kidney I/R (45 min ischemia /24 h reperfusion). At the end of reperfusion period, blood and renal tissue samples were obtained for the serum urea and creatinine (Scr) measurements as well as the tissue antioxidant assays.Results: Induction of I/R and pretreatment with PO extract, increased the level of superoxide dismutase (SOD). There were no significant changes in the levels of MDA, GSH and FRAP among different groups. On the other hand the Scr and serum urea of the I/R and treated groups were elevated compared to the sham group.Conclusion: Purslane did not strongly affect the renal antioxidant status and could not prevent the renal injury following I/R. Probable reason might be the stimulation of immune system by the PO extract.
Authors and Affiliations
Majid Askaripour, Syed Reza Fatemi Tabatabaei, Foruzan Hosseini, Hossein Najafzadeh Varzi
Keywords
Related Articles
- EP ID EP579240
- DOI -
- Views 74
- Downloads 0
VALIDATED STABILITY-INDICATING RP-HPLC ASSAY METHOD FOR AZATHIOPRINE IN PHARMACEUTICAL DOSAGE FORM ACCORDING TO ICH GUIDELINES
Objective: A simple, sensitive, precise and accurate stability-indicating HPLC method has been developed and validated for determination of Azathioprine in bulk drug and in pharmaceutical dosage form in the presence of d...
A NOVEL RAPID RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE QUANTITATIVE ESTIMATION OF BALOFLOXACIN IN TABLETS
Objective: To develop a rapid, accurate, precise and linear Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method and validate as per ICH guidelines for the quantitative estimation of Balofloxacin in tab...
DETERMINATION OF BIOACTIVE CONSTITUENTS OF LEAVES OF CORCHORUS AESTUANS (L.) BY GC - MS ANALYSIS
Objective: To investigate the bioactive constituents of ethanol extract of Corchorus aestuans (L.) using GC-MS technique.Methods: The ethanol extract of leaves were subjected to GC- MS analysis using GC Clarus 500 (Perki...
FORMULATION AND EVALUATION OF TABLET PREPARED BY COAMORPHOUS SYSTEM CONTAINING ANTI-HYPERTENSIVE AND ANTI-HYPERLIPIDEMIC DRUG
Objective: Amlodipine besylate (AML) and Atorvastatin calcium (ATR) belong to biopharmaceutical classification system (BCS) class II (i.e. low solubility and high permeability) which leads to variable bioavailability. He...
A REVIEW ON BIOLOGICAL IMPORTANCE OF PYRIMIDINES IN THE NEW ERA
Pyrimidines occupy an important position in the medicinal world as it has a number of diverse biological properties. Their related fused heterocycles are of interest as potential bioactive molecules. Pyrimidine derivativ...