Effect of quercetin on the number of blastomeres, zona pellucida thickness, and hatching rate of mouse embryos exposed to actinomycin D: An experimental study
Journal Title: International Journal of Reproductive BioMedicine - Year 2018, Vol 16, Issue 2
Abstract
Background: Quercetin is a flavonoid with the ability to improve the growth of embryos in vitro, and actinomycin D is an inducer of apoptosis in embryonic cells. Objective: The aim was to evaluate the effect of quercetin on the number of viable and apoptotic cells, the zona pellucida (ZP) thickness and the hatching rate of preimplantation embryos exposed to actinomycin D in mice. Materials and Methods: Two-cell embryos were randomly divided into four groups (Control, Quercetin, actinomycin D, and Quercetin + actinomycin D group). Blastocysts percentage, hatched blastocysts, and ZP thickness of blastocysts was measured. The number of blastomeres was counted by Hoechst and propidium iodide staining and the apoptotic cells number was counted by TUNEL assay. Results: The results showed that the use of quercetin significantly improved the growth of embryos compared to the control group (p=0.037). Moreover, quercetin reduced the destructive effects of actinomycin D on the growth of embryos significantly (p=0.026). Conclusion: quercetin may protect the embryos against actinomycin D so that increases the number of viable cells and decreases the number of apoptotic cells, which can help the expansion of the blastocysts, thinning of the ZP thickness and increasing the hatching rate in mouse embryos.
Authors and Affiliations
Hamid Reza Sameni, Sara Sadat Javadinia, Manouchehr Safari, Mohammad Hasan Tabrizi Amjad, Nasrin Khanmohammadi, Houman Parsaie, Sam Zarbakhsh
Keywords
Related Articles
- EP ID EP283315
- DOI 10.29252/ijrm.16.2.101
- Views 111
- Downloads 0
Reproductive toxicity of manganese dioxide in forms of micro- and nanoparticles in male rats
Background: Manganese Dioxide (MnO2) has long been used in industry, and its application has recently been increasing in the form of nanoparticle. Objective: The present study was an attempt to assess the effects of MnO2...
Impact of morphine dependency and detoxification by methadone on male’s rat reproductive system
Background: One of the problems that addicts suffer from is decreased libido. Erectile dysfunction has been reported in men using opioids for treatment of heroin addiction. Objective: The study was performed to investiga...
Examining the sexual function and related attitudes among aged women: A cross- sectional study
Background: Sexual function and its subsequent satisfaction are among the most important aspects of women’s life. However, this instinct could be influenced by some factors such as diseases, drug using, aging, and hormon...
Vaginal progesterone on the prevention of preterm birth and neonatal complications in high risk women: A randomized placebo-controlled double-blind study
Background: Preterm birth is the major cause of neonatal mortality and morbidity. Objective: The aim of this study was to evaluate the effect of prophylactic vaginal progesterone on decreasing preterm birth rate and neo...
The effects of multi mineral-vitamin D and vitamins (C+E) supplementation in the prevention of preeclampsia: An RCT
Background: Several studies have reported the uncertain role of multi-minerals and vitamins in the prevention of preeclampsia. Objective: The present study aims to investigate the effect of multimineral-vitamin D supple...