Effect of solvent, time and method of extraction on the amounts of phenolic, flavonoid and the antioxidant activity of the extract of Ixiolirion tataricum (Pall.) Schult. & Schult.f.
Journal Title: Trends in Phytochemical Research - Year 2018, Vol 2, Issue 3
Abstract
Solvent type, extraction time and method are among important parameters in the evaluation of extraction efficiency. The selection of appropriate conditions to increase the extraction efficiency is of great importance. The response surface method (RSM) is a statistical and mathematical technique which can be used to examine the dependencebetween the responses with variables and also to determine the optimal conditions. In this study, the experimental design of general full factorial (GFF) was used using Minitab 17 software. The variables were time (60, 90 and 120 min), solvent (water, methanol and n-hexane) and method (soxhlet and ultrasound-assisted extraction). The main effects and also interaction terms were investigated on amounts of total phenolic content (TPC), total flavonoid content (TFC) and the antioxidant activity (according to IC50, FRAP and BCB) of the extract of Ixiolirion tataricum (Pall.) Schult. & Schult.f.. Under optimal conditions, the best extraction method for the TFC was the ultrasound-based approach, whereas for other responses the soxhlet-based was better. Water was the optimal solvent for TPC, FRAP and BCB, but methanol was found as the best solvent for the determination of TFC and IC50. Also, the extraction time for all of the responses was 90 min. The amounts of TPC, TFC, IC50, FRAP and BCB in the optimum conditions obtained were 22.45 ± 0.60 (mg GAE/g extract), 114.57 ± 1.59 (mg QC/g extract), 0.36 ± 0.08 (mg/mL), 1014.7 ± 12.4 (mmol Fe2+/g extract) and 53.02 ± 0.05, respectively. The R² values for responses were close to unity, which indicates the compatibility between the experimental and the real data. A linear correlation was observed between phenolic compounds and antioxidant activity. Also, the effect of solvent was more important than time and extraction method on the amounts of TPC, TFC, IC50, FRAP and BCB.
Authors and Affiliations
Fatemeh Rabbani; Malihe Samadi Kazemi
Keywords
Related Articles
- EP ID EP680571
- DOI -
- Views 132
- Downloads 0
Nano-encapsulation of thyme essential oil in chitosan-Arabic gum system: evaluation of its antioxidant and antimicrobial properties
In this study Nano–capsules based on chitosan (CS) and Arabic Gum (GA) with Thyme essential oil (TEO) as an active ingredient were prepared using emulsion method. The nano–capsules were characterized by their Encapsulati...
Flavonoids from two Turkish Centaurea species and their chemotaxonomic implications
Centaurea asutro-anatolica Hub.-Mor. and C. kizildaghensis Uzunh., E. Doğan & H. Duman, two indigenous perennial herbs from the Turkish flora, belong to the medicinally important genus Centaurea L. (fam: Asteraceae), whi...
Chemical composition of Theobroma cacao L (Sterculiaceae) and Sorghum bicolor (L) Moench, Syn. Sorghum vulgare Pers (Poaceae)
Theobroma cacao L (sterculiaceae) and Sorghum bicolor (L) Moench, Syn. Sorghum vulgare. Pers (Poaceae) are plants with well-known medicinal properties. Aqueous extracts of the plant blend are being used in the treatment...
Quantification of ursolic acid, correlations and contribution by other traits towards accumulation of ursolic acid in six Ocimum species
Sixteen accessions belonging to six species of Ocimum from Uttar Pradesh (12), Andhra Predesh (3) and Marashtra (1) states of India were screened for ursolic acid and higher oil yields. A considerable amount of genetic v...
Antioxidant properties of various extract from selected wild Moroccan aromatic and medicinal species
Antioxidant properties of various extracts from different wild Moroccan aromatic and medicinal plants (Achillea ageratum L., Salvia officinalis L., Thymus satureoiedes Cosson., Mentha suaveolens subsp. timija (Briq.) Har...