Effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1
Journal Title: Chinese Journal of Blood Transfusion - Year 2022, Vol 35, Issue 11
Abstract
Objective To determine the effect of storage time on the chemokine storage and chemokine scavenging function of erythrocyte atypical chemokine receptor 1(ACKR1). Methods Samples from six bags of red blood cells product, split into two gruoups(10 mL each), were stored in a refrigerator and sampled on day 5 and day 25 during storage, respectively, to measure the concentrations of CCL5, CXCL8 and CCL11 and the ACKR1 chemokine scavenging function of erythrocytes. In addition, 42 erythrocyte products(1 mL each), stored for 5 to 25 days, were sampled to measure the expression of ACKR1 and the concentrations of chemokines CCL5, CXCL8 and CCL11 on the erythrocyte membrane. Results Compared with RBCs stored for 5 days, no difference in the concentration of CCL5(467.7±250.2 pg/mL vs 586.9±209.5pg/mL, P>0.05) and CCL11(122.2±30.3pg/mL vs 125.5 ±32.7pg/mL, P>0.05)were noticed in erythrocyte lysates stored for 25 days, but the concentrations of CXCL8 decreased significantly(42.4±5.3pg/mL vs 24.3± 5.9pg/mL, P<0.05), and the scavenging ability of erythrocyte ACKR1 to chemokines CCL5(2 634.0±730.2pg/mL vs 453.8±257.4 pg/mL, P<0.05), CXCL8(1 117.0±236.6pg/mL vs 306.2±28.3pg/mL, P<0.05) and CCL11 decreased(1 278.0 ±164.5pg/mL vs 467.2 ±50.9pg/mL, P<0.05). Chemokines CCL5, CXCL8 and CCL11 had not been detected on the surface of erythrocyte membrane. There was no significant change in the expression of ACKR1 on the surface of erythrocytes from day 5 to day 25 (P>0.05). Conclusion Erythrocytes are the main places for storing ACKR1 binding chemokines. During the storage, the chemokine scavenging of erythrocyte ACKR1 and some intracellular the ACKR1 binding chemokines are reduced.
Authors and Affiliations
Shihang ZHOU, Ni WANG, Ming LIU, Xiaohua LIANG
Keywords
Related Articles
- EP ID EP738400
- DOI 10.13303/j.cjbt.issn.1004-549x.2022.11.005
- Views 45
- Downloads 0
Influence of blood donation autonomy perception on intrinsic motivation: a moderated mediation model
[Objective] To analyze the mediating pathways of autonomy perception on the intrinsic motivation for blood donation and the moderating mechanism of satisfaction, and to explore the factors that enhance the intrinsic moti...
Effect of high intraoperative plasma dose on the prognosis of patients undergoing blood transfusion during perioperative period
Objective To explore the relationship between the intraoperative plasma transfusion volume, the changes of blood coagulation test values and the clinical prognosis of surgical patients, so as to provide a theoretical bas...
Investigation on the distribution frequency of Rh and Kell blood group antigens in the Uyghur population in Xinjiang and the molecular mechanism of Kell positive individuals
Objective To investigate the distribution frequency and characteristics of Rh and Kell erythrocyte blood group antigens in Uygur population in Xinjiang, and to explore the molecular mechanism of K gene positive patients,...
Residual risk assessment in the window period of nucleic acid testing for transfusion transmitted disease in Hainan
Objective To analyze the nucleic acid testing(NAT) results of voluntary blood donors in Hainan and evaluate the residual risk in the window period of NAT for hepatitis B virus (HBV), hepatitis C virus (HCV) and human imm...
Application of machine learning in predictive analysis of blood usage for liver transplantation surgery
Objective To explore the application of machine learning in scientific and rational blood preparation and predictive analysis for surgical blood usage before liver transplantation surgery. Methods Clinical basic informat...