Effect of sucrose concentration and growth regulators in the in vitro tuberization of Dioscorea alata L. variety Cartagena
Journal Title: Biotecnología Vegetal - Year 2004, Vol 4, Issue 4
Abstract
Among the species of Dioscorea which tubers are used as human food, D. alata L. is one of the most productive and important economically. In this work the effect of sucrose (0, 2, 4, 6, 8,10%) and growth regulators concentration (ANA 0, 0.27, 2.7, 27, 54 μM y 6-BAP 0, 0.22, 2.2, 22, 44 μM) on in vitro tuberization of yam (Dioscorea alata L.) var. Cartagena were evaluated. The nodal segments were established in a MS culture medium supplemented with thiamine 1mg.l-1, myo-inositol 100 mg.l-1, L-cysteine 20 mg.l-1, agar 6 %. The pH was adjusted to 5.7. The cultures were kept under conditions of artificial light (37μM.m-2.s-1). The sucrose concentrations 6 and 8 % favored the production of major number of microtubers and of a lager size compared to the lower concentrations (0, 2 ,4%). The weight of the microtubers increased with the increasing of the concentrations of sucrose and declined with the highest concentration used (10%). The same trend was observed in the total tuber biomass. On the other hand, an evolvement in size and the total tuber biomass on microtuber was observed at highest concentrations of ANA tested (27 y 54 μM). Both, the number and weight of microtubers declined when high concentrations of 6-BAP in the culture medium were used (2.2, 22 y 44).
Authors and Affiliations
Maylen García Corria, Maritza Escalona, Silvio Meneses
Keywords
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