Effect of sucrose in the production of cellulose by Gluconacetobacter xylinus in static culture
Journal Title: Revista MVZ Cordoba - Year 2012, Vol 17, Issue 2
Abstract
Objective. Determine the effect of sucrose on the productivity of BC by Gluconacetobacter xylinus IFO 13693 in static condition. Materials and methods. The synthesis of bacterial cellulose (BC) by Gluconacetobacter xylinus was carried out in a discontinuous static culture at room temperature, in the presence of sucrose as the main carbon source at initial concentrations of 0.8 to 7.6% (p/v). The residual concentrations of BC, sucrose, glucose and fructose were measured every week. The Microcal Origin 6.0®. Software used to determine the kinetics of hydrolysis of sucrose and formation of cellulose and the coefficient of performance of the product Results. In the fourth week the BC values were between 32.5 to 39.5 g/L for the different concentrations of sucrose. The kinetics for the hydrolysis of sucrose fits the Michaelis-Menten model, with a Vmax of 0.0002 mol L-1 h-1 and Km of 0.018 M. The production of BC follows the model proposed by Marx-Figini and Pion, with a value of the slope (kc) between 0.0018 and 0.0024 h-1 for different initial concentrations of sucrose. The yield coefficients have values of 0.8 to 2.4 g of BC produced / g of sucrose consumed. Conclusions. The hydrolysis of sucrose, fructose consumption and glucose is reflected in cellulose synthesis. The hydrolysis of sucrose and production of BC fit the Michaelis-Menten model and the model proposed by Marx-Figini and Pion, respectively. Finally, the performance depends on the concentration of sucrose.
Authors and Affiliations
Rubén Jaramillo L, Ph. D *| Universidad Libre, Facultad de Ciencias de la Salud, Laboratorio de Investigación, Grupo de Investigación en Biomembranas (GIBIOM), Barranquilla, Colombia.Universidad de Sucre, Facultad de Educación y Ciencias, Programa de Biología, Laboratorio de Investigación en Microbiología (LIM), Grupo de Investigación en Biología de Microorganismos (GIBM), Sincelejo, Colombia.Correspondencia: rjaramillo@unilibrebaq.edu.co, Wladimir Tobio J, Ing| Universidad Libre, Facultad de Ciencias de la Salud, Laboratorio de Investigación, Grupo de Investigación en Biomembranas (GIBIOM), Barranquilla, Colombia.Universidad del Atlántico, Programa de Ingeniería Química, Facultad de Ingeniería, Barranquilla, Colombia., José Escamilla M, Ph. D| 4Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Ciudad Universitaria 04510, México, D.F., México.
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