EFFECT OF SUPPLEMENTATION WITH ALANINE AND GLICYNE ON THE INITIAL CLEAVAGES OF BOVINE EMBRYOS PRODUCED IN VITRO
Journal Title: Revista MVZ Cordoba - Year 2007, Vol 12, Issue 2
Abstract
Objective. To determine the effect of supplementation with alanine and glicyne in vitro culture systems on the cleavage rate of bovine embryos under conditions of high (20%) and lower (7%) oxygen tension. Materials and methods. The embryos were produced from oocytes matured in vitro in M-199 supplemented with hormones and inseminated with cryopreservated semen; the zygotes were cultivated in CR1-AA medium. At culture moment alanine and glicyne were added (5 mm and 10mM final respectively). Four (4) treatments were used (T1: amino acids and low oxygen tension; T2: amino acids and high oxygen tension; T3: non amino acids and low oxygen tension; T4: non amino acids and high oxygen tension). The cleavage was evaluated to 48 hpi (hours post insemination) being evaluated the number of embryos cleavaged on the total of culture embryos and the development stage (non cleavaged, 2, 4, 5-8 cells). The statistical program STATISTICA version 5.0 was used. Results. Embryos rate of 5-8 cells in the treatment one regarding the other 3 ones were higher (p = 0.007). Conclusions. The amino acids alanine, glicyne and in low oxygen tensions are fundamental for the initial cleavages because the proportion of competent embryos increases up to 48 hpi.
Authors and Affiliations
Catalina Arias L*| Universidad de Antioquia. Facultad de Ciencias Agrarias. Grupo Fisiología y Biotecnología Animal. Carrera 75 No 65-87. Bloque 46 Lab. 202. Medellín - Colombia.Correspondencia: mariak_53@yahoo.es, Zoot, Tatiana Ruiz C| Universidad de Antioquia. Facultad de Ciencias Agrarias. Grupo Fisiología y Biotecnología Animal. Carrera 75 No 65-87. Bloque 46 Lab. 202. Medellín - Colombia., Ph.D, Martha Olivera A| Universidad de Antioquia. Facultad de Ciencias Agrarias. Grupo Fisiología y Biotecnología Animal. Carrera 75 No 65-87. Bloque 46 Lab. 202. Medellín - Colombia., Dr. Sci. Agr, Ariel Tarazona M| DPA/FCA/BIOGEM Universidad Nacional de Colombia, Sede Medellín, Colombia., M.Sc
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