Effect of temperature, pH, substrate (Starch) and glucose on stability of α-amylase from Bacillus licheniformis ATCC 6346
Journal Title: Scholars Academic Journal of Pharmacy - Year 2014, Vol 3, Issue 6
Abstract
Thermostable α-amylases (1,4-α-D-glucan glucanohydrolase; E.C.3.2.1.1) from Bacillus species are of great industrial importance in the production of corn syrup or dextrose. The present study was aimed to determine the effect of temperature, pH, substrate and glucose on stability of α-amylase from Bacillus licheniformis ATCC 6346. To determine the effect of temperature and pH on the stability of α-amylase this was pre-incubated at different temperatures and pHs. The enzyme pre-incubated with starch and glucose separately at 85oC to determine the effect of substrate and end-product on the stability of α-amylase. Crude -amylase retained 60% of the original activity when it was pre-incubated at 85oC for 10 min. This enzyme was more stable at pH 9.0 than other pH values (7.0 and 8.0) and no significant important was observed with addition of substrate (starch). Presence of glucose slightly increased the stability of -amylase. There was no significant improvement in the stability of -amylase was observed with the addition of starch however presence of glucose slightly increased the stability of the α-amylase. Keywords: Enzyme activity, enzyme stability, α -amylase, half- life, starch
Authors and Affiliations
A. Vengadaramana ,, S. Balakumar ,, Vasanthy Arasaratnam
Keywords
Related Articles
- EP ID EP378107
- DOI -
- Views 61
- Downloads 0
Self-emulsifying Drug Delivery System (SEDDS): An Approach for Solubility, Dissolution and Bioavailability Enhancement of Furosemide
Abstract:Furosemide is a high-ceiling (loop) diuretic, which is extensively used in the treatment of hypertension and oedema associated complications. However, when delivered orally, furosemide is poorly water-soluble an...
Clinical Pharmacist Role in Intravenous Administration Errors: A Review
Potential activation of clinical pharmacist role is of great importance in reducing the IV administration errors which are well-known problem in hospitals. IV errors have been shown to be frequent and serious. The purpos...
Study of Influence of Povidone and Sodium Lauryl Sulphate on Performance of Mouth Dissolving Tablets of Mirtazapine
Mouth dissolving tablets disintegrate and/or dissolve in saliva immediately when they are placed in mouth. Their rate of disintegration and dissolution are highly influenced by the formulation and method of manufacture....
Design, Synthesis and Pharmacological Evaluation of 2, 5 -Disubtituted-1, 3, 4-Oxadiazole Derivatives as Noval Antibacterial and Antifungal Agent
Abstract: The 1, 3, 4-Oxadiazole nucleus is associated with various pharmacological activities and are potential linker moieties. The Schiff base derivatives of 5-arylsubstituted-1, 3, 4-oxadiazole-2-amine were synthesiz...
Extraction, Isolation and Antimicrobial Activity of Crude and Purified Ferritin extract from seeds of Soyabean (Glycine max (L.) Merr. )
The present study aimed to isolate, purify and evaluate the antimicrobial activity of ferritin extracted from Soyabean (Glycine max (L.) Merr. ) against Gram-negative microorganisms (Escherichia coli, Pseudomonas aerugin...