Effect of The Ethylene Inhibitor “Agno3”, Vitamin B9 “Folic Acid” And Thiol Compound “GSH” On in Vitro Propagation of Sideritis Syriaca L. Subsp. Syriaca (Hellenic Mountain Tea of The Crete Island)
Journal Title: JOURNAL OF ADVANCES IN BIOTECHNOLOGY - Year 2019, Vol 8, Issue 0
Abstract
The aim of this research was to study the effect of the ethylene inhibitor “silver nitrate (AgNO3)” and vitamin B9 "folic acid" in different concentrations combined with cytokinin BA as well as the antioxidant thiol compound “L-glutathione reduced (GSH)” in different concentrations simultaneously with auxins (IBA+NAA) on micropropagation efficiency of the endemic Sideritis syriaca L. subsp. syriaca (Hellenic mountain tea of Crete/ Malotira) using shoot tip explants. The culture medium used was the MS supplemented with 30 g/l sucrose. The simultaneous application 5 ?M AgNO3 + 2.2 ?M BA promoted best the initial shoot induction stage exhibiting 4.5 shoots/ explant and 100% shoot multiplication (5 weeks). Folic acid applied at 0.25 mg/l in combination with 0.5 mg/l BA exhibited the highest shoot multiplication percentage (90%) (4 weeks). GSH at 10 ?M with 10.7 ?M NAA + 4.92 ?M IBA gave the greatest root length (13.68 mm), at 25 ?M caused a 3-fold increase in rooting (90%) and 250 ?M GSH raised by 20% shoot multiplication (80%). An 89% final survival rate of rooted microplants to ex vitro unheated greenhouse conditions was recorded within 8 weeks period during mid-late spring. Thus, the acclimatization and hardening process was successfully completed.
Authors and Affiliations
Virginia Sarropoulou, Eleni Maloupa
Keywords
Related Articles
- EP ID EP653914
- DOI 10.24297/jbt.v8i0.8385
- Views 103
- Downloads 0
No evidence of association between apo E polymorphism and BMI and lipids profile in Turkmen population
Apolipoprotein E (apoE) is a glycoprotein that plays an essential role in lipid transport and metabolism.The present study purposed to determine the distribution of apoE alleles in a Turkman population and its associatio...
Isolation and Optimization of Lipid Production from Drechslera Sp. and Feasibility of using Orange Peel as a Substrate for Growth
This study deals with isolation and optimization of soil origin fungus Drechslera sp. with valued unsaturated fatty acids using orange peel as a substrate. Accordingly, to maximize oil production, various parameters were...
Improving the anti-oxidation of glucose oxidase with computer-aided structure optimization
Glucose oxidase (GOD) can be inactivated by hydrogen peroxide (H2O2) produced during glucose oxidation; thus, H2O2 is a competitive inhibitor of GOD. In this mechanism, methionine (Met), a sulfur-containing amino acid, c...
Micropropagation and ex situ conservation of Silene fabaria (L.) Sm. in Sibth. & Sm. subsp. domokina Greuter (Caryophyllaceae); an important endemic plant in Greece with medicinal and ornamental value
Silene species (Caryophyllaceae) are sources of important secondary metabolites with extensive use in traditional medicine and potential applications as ornamentals. The present study was conducted to assess the regenera...
Cranberry Extract Suppressive Effect on Secretion of Inflammatory Cytokines by the Monocytic Cells
The proanthocyanidin rich fraction of cranberry (NDM) has long been speculated to possess the capacity to suppress the production of inflammatory mediators by macrophages. The objective of this investigation was to exami...