Effect of the gynosaponin on methane production and microbe numbers in a fungus-methanogenco-culture
Journal Title: Journal of Animal and Feed Sciences - Year 2011, Vol 20, Issue 2
Abstract
This study investigated the effect of saponins gypenoside (gynosaponins) on methane production and microbe numbers in a co-culture of a fungus, Piromyces sp. F1, and a methanogen, Methanobrevibacter sp.. Two co-culture systems were used: with methanogen (co-culture I) and without methanogen (co-culture II; methanogen growth inhibited by chloramphenicol). Each co-culture system was treated with 0, 50, 100 or 200 mg gynosaponins/l culture medium. Gas production, methane concentration and volatile fatty acid concentration (VFA) were measured for each treatment group. The numbers of anaerobic fungi and methanogen were quantified by real time PCR. The results showed that, compared with the control, gynosaponin significantly reduced the gas production, methane concentration, methane to TVFA ratio (total volatile fatty acid), TVFA concentration, number of fungi (except for 50 mg dose of gynosaponin in co-culture I) and number of methanogens. Methane was not detected in co-culture II. The individual VFAs proportion of TVFA were not affected by gynosaponins in either of the co-cultures. The pH was higher in both co-cultures than that of the control (P<0.01). These data suggest that gynosaponins has the potential for being used as feed additive to modulate the ruminal fermentation, inhibit the methanogen growth and reduce methane production.
Authors and Affiliations
X. F. Wang, S. Y. Mao, J. H. Liu, L. L. Zhang, Y. F. Cheng, W. Jin, W. -Y. Zhu
Keywords
Related Articles
- EP ID EP74864
- DOI -
- Views 113
- Downloads 0
Responses of mammary amino acid metabolism and aminopeptidase N gene expression to duodenal soyabean small peptides and infusion of free amino acids in lactating goats
The duodenal perfusion technique was used to determine whether small peptides were more effective than free amino acids (FAA) for milk protein synthesis and to investigate the effects of infusing soyabean small peptides...
Choosing a feed evaluation system - NRC vs INRA- to formulate rations for growing goats using minimum cost linear programming
The influence of feed evaluation system and type of forage in the rations for three, eight and twelve months old goats optimized through minimum cost linear programming was studied. The rations included one of three fora...
Can silver nanoparticles affect the mineral content, structure and mechanical properties of chicken embryo bones?
investigated whether hydrocolloids of silver nanoparticles (nano-Ag) are deposited in chicken bones during embryogenesis and to what extent they can affect biochemical characteristics, mineral content, structure, and mec...
Genetic relationships among time of egg formation, clutch traits and traditional selection traits in laying hens
In a population of Rhode Island White hens heritability of egg formation, clutch characters and traditional selection traits as well as the genetic and phenotypic correlations between them were estimated via multitrait a...
The influence of supplementing heifer diets with Saccharomyces cerevisiae yeast on the activity of polysaccharidases in the rumen
The effect of live cells and metabolites of the yeast, Saccharomyces cerevisiae, in the diet on the activity of polysaccharidases in the rumen of heifers was examined. The experiment was carried out on three rumen-fistul...