Effect of the inoculation density in Coffea arabica L. cv. ‘Caturra rojo’ somatic embryos germination in RITA ® Temporary Immersion System
Journal Title: Biotecnología Vegetal - Year 2014, Vol 14, Issue 2
Abstract
The development of somatic embryogenesis of coffee (Coffea spp.) in liquid culture medium is a viable alternative for the propagation of these species. The use of liquid culture medium and temporary immersion systems could increase the germination of somatic embryos and improve the quality of plants. The objective of this work was to determine the effect of inoculation density on germination of somatic embryos of Coffea arabica L. cv. ‘Caturra rojo’ in temporary immersion systems RITA ® . It were used as inoculum densities 40, 50, 60, 70 and 80 somatic embryos per RITA ® . After 90 days of culture the number of somatic embryos germinated, hyperhydricity symptoms, number of true leaves, length and root development was quantified. With inoculum density of 70 somatic embryos per RITA ® , it was obtained a highest germination percentage (60%) with good leaf development and length of the plants.
Authors and Affiliations
Raúl Barbon , Hien Nguyen Thi 1, Alina Capote , Manuel de Feria , Elisa Quiala , Anabel Pérez
Keywords
Related Articles
- EP ID EP683562
- DOI -
- Views 130
- Downloads 0
Novel explant for somatic embryogenesis in Sorghum bicolor (L.) Mohen
This work was carried out with the objective to form somatic embryos of sorghum, cv. ‘CIAP 132R-05’ starting from callus obtained from sections of in vitro shoots. For the formation of callus, different concentrations o...
n vitro multiplication of Colocasia esculenta clone ‘INIVIT MC-2001’ in semi-automated culture systems
One alternative to increase yields in the taro (Colocasia esculenta) culture, could be the application of biotechnological methods, especially semi-automated systems. The new clone ‘INIVIT MC-2001’ with high productive...
In vitro conservation of sweet potato clones under minimal growth conditions
Nodal segment of INIVIT B-88, CEMSA 78-354, INIVIT B-90-510 and Cautillo sweet potato in vitro plants clone were conserved for one year in culture medium with abscisic acid 10.0 mg.l- with high survival and recovery perc...
Effect of parameters involved in the transformation via Agrobacterium tumefaciens in the regeneration of Phaseolus vulgaris L. cultivars ‘ICA Pijao’ and ‘BAT 93’
Phaseolus vulgaris is a crop widely used in human consumption. Its production is affected by the incidence of biotic and abiotic factors. Genetic transformation provides the possibility of reducing these affections, bu...
Callogenesis and rhizogenesis of Viola odorata L.
Viola odorata L. is a medicinal plant and source of different secondary metabolites. It has been proven that callus culture can be used as an effective method for multiplication of medicinal plants. The aim of this paper...