Effect of Two Different Hydrating Solutions on Antibacterial Property of White Mineral Trioxide Aggregate Against Enterococcus Faecalis - An in Vitrocomparative Study

Journal Title: IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) - Year 2017, Vol 16, Issue 3

Abstract

Background: Antimicrobial activity of root canal materials may help to eliminate residual microorganisms unaffected by the effects of both chemo-mechanical preparation and intracanal medication. Mineral trioxide aggregate (MTA), is one such bioactive material that has potential antimicrobial property. The principle aim of this study was to evaluate the antibacterial properties of ProRoot WMTA against E. faecalis and to compare the antibacterial effect of WMTA when mixed with 2% Chlorhexidine (CHX), and Phosphate Buffered Saline (PBS), as a substitute for sterile water against Enterococcus faecalis (E. faecalis). Method: ProRoot WMTA was divided into three groups one for each hydrating agent. The antimicrobial effect was determined by placing freshly mixed MTA samples in wells created in trypticase soy agar (TSA) plates inoculated with Enterococcus faecalis. These plates were maintained at room temperature for 2 hours for prediffusion of the materials and incubated at 370C for 24 hours and zones of inhibitions were recorded. Results: The samples of ProRoot WMTA irrespective of the mixing agent showed antimicrobial activity against E. faecalis. Higher mean zone of inhibition was shown by WMTA/ CHX followed by WMTA/PBS and WMTA/Sterile Water. The difference between the three groups were found to be statistically significant with P value <0.001. Conclusion: Phosphate buffered saline (PBS) and 2% Chlorhexidine enhanced the antibacterial efficacy to inhibit E. faecalis and can be recommended to be used as mixing agent for WMTA as substitute to sterile water provided by manufacturer.

Authors and Affiliations

Dr. Venkatesh Babu NS, Dr Ganga. A. , Dr. Parin V. Bhanushali

Keywords

Related Articles

Dabigatran (Pradaxa) Induced Liver Injury (Case Presentation)

Dabigatran is a novel antithrombin anticoagulant that was approved by the FDA in 2010 to be used for the prevention of stroke and venous embolism in patients with chronic atrial fibrillation. In this article, we present...

Peeling skin syndrome in a 3 year old female child

We report a case of 3 year old Hindu female child who presented to skin OPD with complaint of spontaneous peeling of skin from complete body which was insidious in onset and progressive in nature, started since 10th day...

Idiapathic Hypereosinophilic Syndrome with Rheumatoid Arthritis

Idiopathic Hypereosinophilic Syndrome is defined as1) sustained eosinophilia more than 1500/mm3 .2)absence of other causes of eosinophilia.3) multiorgan involvement. Very few case reports have been reported in the past o...

Biosimilars in rheumatology,

The use of biologic therapy has revolutionarized treatment of many chronic diseases including several rheumatological disorders. However , biological agents are expensive and unaffordable. The follow-on generic products,...

Comparative Evaluation of Elisa and Rapid Screening Techniques for the Diagnosis of HCV amongst Suspected Viral Hepatitis Cases Attending Tertiary Care Hospital, Kolkata

HCV is a blood borne virus that attacks the liver and can cause chronic hepatitis, liver cirrhosis (27%) and liver cancer (25%). So detection of HCV infection in suspected patients by a reliable rapid method is a need of...

Download PDF file
  • EP ID EP592523
  • DOI 10.9790/0853-1603024651
  • Views 78
  • Downloads 0

How To Cite

Dr. Venkatesh Babu NS, Dr Ganga. A. , Dr. Parin V. Bhanushali (2017). Effect of Two Different Hydrating Solutions on Antibacterial Property of White Mineral Trioxide Aggregate Against Enterococcus Faecalis - An in Vitrocomparative Study. IOSR Journal of Dental and Medical Sciences (IOSR-JDMS), 16(3), 46-51. https://europub.co.uk/articles/-A-592523