Effect of ultrasound on the production of Carvone as a secondary metabolite in callus derived from Bunium persicum Boiss

Journal Title: Journal of Herbal Drugs - Year 2011, Vol 2, Issue 2

Abstract

Background & Aim: Medicinal plants are most valuable and useful sources as productive factors of drugs. Production and extraction of secondary metabolites have a huge economical importance in recent years. Carvone is a compound in Bunium persicum fruit and shoot and, it is one of the wide range varieties of valuable and useful secondary metabolites. Due to limited crop production, using appropriate stimulating factors, such as ultrasound is an efficient way in the production of secondary metabolites in plant cell culture. Experimental: In this study, callus in suspension environment influenced by treatments once or twice, with times 0, 5, 20, 35, 50 and 240 seconds by means of ultrasonic bath with a frequency of 38.5 kHz and constant power examined. After extraction of Carvone, the amount of this secondary metabolite was measured by High performance liquid chromatography (HPLC) method. Results & Discussion: Stimulating effect of ultrasound increased the synthesis of Carvone dramatically. According to the observations, combination of hormone with using the bath for 2 times and each time for 35 seconds, created highest rate of Carvone. By contrast, controlling the all cases showed the lowest level of Carvone. Industrial and practical recommendations: Ultrasound irradiation can act as a potent abiotic elicitor to induce the defense responses of plant cells and to stimulate secondary metabolite production in plant cell cultures.

Authors and Affiliations

Mahmood Atrashi; Elham Tavokoli Dinanie; Mohammad Taghi Darzi; Javad Hashemi; Shirin Rozbeh; Amir Masumi

Keywords

Related Articles

Study of phytohormones effects on UV-B stress seeds of thyme species

Background & Aim: Thymus vulgaris L. and Thymus daenensis Celak (Thyme), members of the family Lamiaceae, are widely used in Iranian folk medicine. The aim of this research was to study how salicylic acid (SA), gibber...

Antibacterial activity of the essential oils of endemic plants

Background & Aim: Secondary metabolites with bioactive and functional properties in essence of aromatic plants can be useful for improvement of quality and persistence of food as natural preservative. The aim of this st...

Evaluation of plant pigments existence in medicinal plant, sumac (Rhus coriaria L.) at two different seasons

Background & Aim: The pigments inside the leaf chloroplasts receive sunlight energy and transport it to photosynthesis reaction center. The most important of these pigments is chlorophyll. In this study, the pigments exi...

Chemical Composition of the Volatile oils of three Lamiaceae species growing wild in North of Iran

Background & Aim: The Lamiaceae or Labiatae (commonly referred to as the mint or deadnettle family) are an exceedingly large and widespread of flowering plants (Angiospermae). Most member of Lamiaceae plants are used...

Effect of jasmonic (JA) and salicylic acid (SA) on phytochemical properties of Salvia officinalis L.

Background & aim: Sage (Salvia officinalis L.) is a perennial medicinal herb which belongs to Lamiales order and Lamiaceae family. This plant has economical value for four years. Nowadays the use of plant growth regulato...

Download PDF file
  • EP ID EP23352
  • DOI -
  • Views 411
  • Downloads 22

How To Cite

Mahmood Atrashi; Elham Tavokoli Dinanie; Mohammad Taghi Darzi; Javad Hashemi; Shirin Rozbeh; Amir Masumi (2011). Effect of ultrasound on the production of Carvone as a secondary metabolite in callus derived from Bunium persicum Boiss. Journal of Herbal Drugs, 2(2), -. https://europub.co.uk/articles/-A-23352