Effect of zinc on galactose-induced cell apoptosis in human lens epithelial cells
Journal Title: Guoji Yanke Zazhi - Year 2018, Vol 18, Issue 10
Abstract
AIM:To study the effect of zinc on galactose-induced cell apoptosis in human lens epithelial cells(HLEC). <p>METHODS:HLEC cell line SRA01/04 cells were cultured in DMEM medium and divided into six groups: control group, galactose treatment group, zinc supplement group, zinc supplementation combined with galactose treatment group, zinc deficiency group, zinc deficiency combined with galactose treatment group. The cell viabilities were assayed by MTT, cell morphology and apoptosis were detected by fluorescence microscope and flow cytometry, respectively. <p>RESULTS:The cell viabilities induced by galactose(0, 25, 50, 75, 100, 125mmol/L)were(100.0±5.4)%,(97.5±3.2)%,(91.3±5.3)%,(93.4±0.6)%,(86.6±1.4)% and(83.5±0.4)%, respectively. When the concentration of galactose was 100 and 125mmol/L, cell viability was significantly decreased, compared with the untreated cells(<i>P</i><0.05). Fluorescence microscopy results showed that the cell nucleus remained uniformly stained in control group and zinc supplement group. Nuclear shrinkage, a typical apoptotic morphology, was visible in some cells in the galactose treatment group, zinc supplementation combined with galactose treatment group, zinc deficiency group and zinc deficiency combined with galactose treatment group. The cell apoptosis in the six groups were(1.5±0.1)%,(7.1±0.2)%,(1.4±0.1)%,(4.4±0.2)%,(5.5±0.2)% and(15.8±0.3)%, respectively. The cell apoptosis were significant increased in galactose treatment group, zinc supplementation combined with galactose treatment group, zinc deficiency group and zinc deficiency combined with galactose treatment group, compared with the control group(<i>P</i><0.05), and those of zinc supplementation combined with galactose treatment group were significant decreased, and zinc deficiency combined with galactose treatment group were increased(<i>P</i><0.05), compared with the galactose treatment group. <p>CONCLUSION:Zinc supplementation protects human lens epithelial cells against apoptosis induced by galactose and may have an inhibition effect on cataract formation.
Authors and Affiliations
Yi Jia
Keywords
Related Articles
- EP ID EP524029
- DOI 10.3980/j.issn.1672-5123.2018.10.04
- Views 118
- Downloads 0
Effect of blue light and white light on proliferation of ARPE-19 cells detected by CCK-8
AIM: To investigate the effects of blue light and white light on the proliferation of human retinal pigment epithelial cell line(ARPE-19)at different times, and lay a foundation for further detecting the changes of relat...
Identification of pathogenic nonsporulating molds in eyes by internal transcribed spacer sequences alignment
AIM: To identify the species and genus of pathogenic nonsporulating molds(NSM)by internal transcribed spacer(ITS)sequences alignment, and reveal the biodiversity of NSM in Hainan Island with tropical climate. <p>METHODS:...
Research advances of endoplasmic reticulum stress in high glucose environment and its related ophthalmic diseases
Endoplasmic reticulum(ER)is a place where it folds and synthesizes the proteins. ER stress was induced when a variety of physiological and pathological factors happened, under which the protein misfolding occurred, the u...
Ocular surface changes and risk factors in patients with type 2 diabetes mellitus
AIM: To analyze ocular surface changes and risk factors in patients with type 2 diabetes mellitus(T2DM). <p>METHODS: Totally 118 cases of T2DM patients(236 eyes)admitted from May 2017 to May 2018 were selected as T2DM gr...
Study progress on the relationship between obstructive sleep apnea hypopnea syndrome and glaucoma
Glaucoma is a progressive optic neurodegenerative disease with specific characteristics of structural optic nerve head(ONH)and with changes in the inner retinal layer(ganglion cell complex)along with the presence of corr...