Effective health management in shrimp hatcheries: use of loop-mediated isothermal amplification (LAMP) for the rapid detection of Philippine isolates of Penaeus monodon-type baculovirus (MBV
Journal Title: Animal Biology & Animal Husbandry - International Journal of the Bioflux Society - Year 2011, Vol 3, Issue 1
Abstract
Effective health management in hatcheries is a crucial step towards the prevention of disease outbreaks during the early life stages of the shrimp. Early and sensitive detection of the different pathogens ensure that proper measures are undertaken before massive mortality occurs. In this study,the loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of the Penaeus monodon-type baculovirus (MBV), a viral pathogen that is common in shrimp hatcheries. The optimized time and temperature conditions for LAMP were 60 min and 63[sup]o[/sup]C, respectively. The assay was highly specific for MBV and did not amplify other shrimp viruses including white spot syndrome virus, infectious hypodermal and hematopoietic necrosis virus (IHHNV) and hepatopancreatic parvovirus (HPV). The sensitivity threshold of LAMP was 10 pg of DNA ml[sup]-1[/sup]or 10 fg of the genomic DNA per LAMP reaction and was 10 times more sensitive than conventional PCR in detecting MBV from infected shrimp postlarvae. As a possible application for field use, both LAMP and conventional PCR were able to detect the virus from tissues of infected shrimp postlarvae. These results demonstrate that LAMP could be used as a simple and sensitive method for the rapid field diagnostic tool for early detection of Philippine isolates of MBV in shrimp hatcheries.
Authors and Affiliations
Christopher Caipang, May Sibonga, Jane Geduspan, Mary Apines-Amar, Adelaida Calpe
Keywords
Related Articles
- EP ID EP97625
- DOI -
- Views 153
- Downloads 0
Nutritional evaluation of poultry by-product meal in broiler chickens
This experiment was conducted to determine the apparent protein digestibility, metabolizable energy (AME) and metabolizable energy correction of zero nitrotion (AME[sub]n[/sub]) of poultry by-product meal (PBPM) from two...
Mitigation of cimetidine induced testicular toxicity in mice by Kaempferia parviflora Wall. Ex Baker rhizome extract
Kaempferia parviflora Wall. Ex Baker is a herb belonging to the family Zingiberaceae, its rhizomes were used as tea or wine and well known as Thai ginseng. This present study is to evaluate the mitigative effect of aque...
Growth response and carcass characteristics of Japanese quail to Mentha piperita plant supplementation
In current study, the effect of Mentha piperita plant (MPP) supplementation on performance and carcass characteristics in Japanese quail was investigated. A total of 180 quails (male and female) were carried out in compl...
Contributions to the knowledge of the Romanian from the western Dacian Basin: The molluscs’ fauna from Răcarii de Jos (Dolj County).
In the western Dacian Basin, the Romanian age starts with a fauna comprised of carved unionidae and robust viviparidae, which appear in numerous crops in Mehedinţi, Dolj and Gorj Counties. The lamellibranchiate species f...
Polyculture of white shrimp, Litopenaeus vannamei and milkfish, Chanos chanos as a strategy for efficient utilization of natural food production in ponds
Both shrimps and milkfish are important aquaculture species in the Philippines. In the present study, we described a modified extensive polyculture method for white shrimp, Litopenaeus vannamei and miklkfish, Chanos chan...