Effectiveness of electrolyzed water on the inactivation of Listeria monocytogenes in letucce (lactuca sativa L.)
Journal Title: Revista MVZ Cordoba - Year 2004, Vol 9, Issue 2
Abstract
The effectiveness of electrolyzed oxidizing water for inactivating L. monocytogenes in suspension and inoculated in lettuce was evaluated. An electrolytic cell for the production of EO water was built and a solution of 5% NaCl was used. The EO water produced had a residual chlorine concentration of 29 parts per million (ppm) and pH 2.83. Ten strains of L. monocytogenes isolated from processed chicken (109 CFU/ml) were inoculated on 9 ml of EO water or 9 ml of deionized water (control) and incubated at 15°C for 5, 10, 15 and 20 min. The surviving population of each strain was determined on Columbia agar. An exposure time of 5 min reduced the populations by approximately 6.6 log CFU/ml. The most resistant strains to hypochlorite (NaOCl) were selected and used in a strain mixture (9.56 log CFU/ml, 109UFC/ml approximately) for the inoculation of 35 lettuces by the dipinoculation method using distilled water as control. The population mean of L. monocytogenes after treatment with EO water and distilled water was reduced by 3.92 and 2.46 log CFU/ml respectively (p= 0.00001). EO water and 6% acetic acid (vinegar) were combined for improve the EO water effect on L. monocytogenes inoculated in lettuce, the effectiveness of this combination were examined. The results showed that there was a synergistic effect of both antimicrobial agents (population reduction by 5.49 log CFU/ml approximately) on the viability of L. monocytogenes cells.
Authors and Affiliations
Casadiego P| Laboratorio de Microbiología de Alimentos, Departamento de Física Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de Microbiología. Bogotá D.C., Cuartas R| Laboratorio de Microbiología de Alimentos, Departamento de Física Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de Microbiología. Bogotá D.C., Mercado M| Laboratorio de Microbiología de Alimentos, Departamento de Física Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de Microbiología. Bogotá D.C., J Carrascal AK*| Laboratorio de Microbiología de Alimentos, Departamento de Física Pontificia Universidad Javeriana, Facultad de Ciencias, Departamento de Microbiología. Bogotá D.C.Correspondencia:E-mail:acarrasc@javeriana.edu.co
Keywords
Related Articles
- EP ID EP3008
- DOI -
- Views 431
- Downloads 24
Isolation and serotyping of Salmonella sp. in ponds with captive Crocodylus intermedius and testudines in Villavicencio – Colombia
Objective. To determine the presence of Salmonella sp., in aquatic environment of Crocodylus intermedius and testudines at the Estación de Biología Tropical Roberto Franco (EBTRF). Materials and methods. Standard methodo...
Reproductive performance of bocachico Prochilodus magdalenae induced twice in the same year
Objective. To evaluate the reproductive performance of hormonally induced bocachico with carp pituitary extract (CPE) twice in the same year. Materials and methods. 23 females, in their final maturation phase, were induc...
Random regression models for milk, fat and protein in Colombian Buffaloes
Objective. Covariance functions for additive genetic and permanent environmental effects and, subsequently, genetic parameters for test-day milk (MY), fat (FY) protein (PY) yields and mozzarella cheese (MP) in buffaloes...
Colonization of arbuscular mycorrhizae in Bothriochloa pertusa (L) A. Camus pasture
Objective. Evaluate in situ the colonization of arbuscular micorrhizae, in grass roots colosuana in function of the physical and chemical characteristics of the soil, in cattle farms of four agroecological areas of the s...
Clinical and histopathology evaluation of cutaneous Pythiosis in calves the department of Cordoba, Colombia
Objective. Evaluate and characterize the clinical and histopathological aspects of cutaneous Pythiosis in calves the department of Córdoba, Colombia. Materials and methods. A descriptive, non-probabilistic, convenience...